Fused pyrimidin-4(3H)-ones as antiallergy agents

ABSTRACT

Fused heterocyclic ring systems in which a quinoline or a pyridine component is &#34;fused&#34; to a pyrimidine having a 2-methyl, 2-ethyl, or 2-acetyl group and a 4-keto group, and to similar ring systems in which a quinoline, a naphthalene or a pyridine component is &#34;fused&#34; to a pyrimidine having a 2-carboxy group and a 4-keto group, derivatives, and pharmaceutically-acceptable cationic salts thereof, and their use as antiallergy agents, and intermediates therefor.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a continuation-in-part of our copending application, Ser. No. 444,138 filed Feb. 20, 1974, and now abandoned which in turn is a continuation-in-part of application Ser. No. 351,025, filed Apr. 13, 1973, and now abandoned.

BACKGROUND OF THE INVENTION

This invention relates to fused heterocyclic ring systems in which a quinoline, a naphthalene or a pyridine ring system is fused to a 2-methyl-, 2-ethyl, or 2-acetyl pyridimidine-4(3H)-one or to a pyrimidine-2-carboxylic acid 4(3H)-one or a derivative thereof, and their use as anti-allergy agents. More particularly, it relates to 2-alkylpyrimido[4,5-b]quinolin-4-(3H)-ones, 2-alkylpyrido[4,5-b]pyrimidon-4(3H)-ones wherein alkyl is methyl or ethyl, and the corresponding 2-acetyl derivatives, pyrimido[4,5-b]quinolin-4(3H)-ones-2-carboxylic acids, benzo[g]quinazolin-4(3H)-one-2-carboxylic acids, and pyrido[2,3-d]pyrimidin-4(3H)-one-2-carboxylic acids; esters, amides, and pharmaceutically-acceptable salts thereof which are useful as inhibitors of allergic reactions, and especially of allergic bronchial asthma.

A number of pyrimido[4,5-b]quinolines (1,3-diazo-acridines) are described in the art (J. Chem. Soc., 727 (1927); J. Hetero. Chem. 7, 99 (1970); J. Am. Chem. Soc. 78, 5108 (1956); and J. Chem. Soc., 552 (1948). However, none of them contain a carboxy group or functional derivative thereof, i.e., an ester, amide, acid chloride, with the exception of 2,4-dihydroxy-pyrimido[4,5-b]quinoline-5-carboxylic acid, its methyl ester, amide and acid chloride; 1,3-dimethyl-1,2,3,4-tetrahydro-pyrimido[4,5-b]quinolin-2,4-dione-5-carboxylic acid methyl ester; and 10-methyl-2,3,4,10-tetrahydro-pyrimido[4,5-b]quinolin-2,4-dione-5-carboxylic acid and its methyl ester. The products were investigated as potential riboflavin antagonists, Taylor et al., J. Am. Chem. Soc. 78, 5108 (1956) describe 2-methylpyrimido[4,5-b]quinolin-4(3H)-one. No 2-carboxy substituted derivatives are described in the literature.

The known pyrimido[4,5-b]quinolin-4(3H)-one when tested by the passive cutaneous anaphylaxis (PCA) test described herein affords only 33% protection in rats at 3.0 mg./kg. via the intravenous route of administration. It is, relative to the compounds of this invention, of marginal value as an anti-allergy agent.

In the benzo[g]quinazolin-4(3H)-one series of compounds of formula II, no analogs wherein a carboxy group (or an ester or amide thereof) is attached directly to the nucleus appear to have been described in the art. However, 2-carbethoxymethylbenzo[g]quinazolin-4(3H)-one is described by Reid et al., Ber. 96, 1216 (1963). No utility is reported for the compound It afforded 49% protection in the PCA test when administered intravenously at 3.0 mg./kg. Upon acid hydrolysis it undergoes facile decarboxylation at room temperature to 2-methylbenzo[g]quinazolin-4(3H)-one (Reid et al., Ber. 95, 3042, 1962) which shows little or no activity in the PCA test.

Benzo[g]quinazolin-4(3H)-one, the compound related to formula II, is reported in Indian Pat. No. 74,146, March 2, 1963 [C.A. 60, 1773f, 1964] to be active as a bronchodilator. When tested in the PCA test described below it was observed to provide 31% protection in rats at 3.0 mg./kg. by the intraveneous route of administration. It is, at best, of marginal value in the treatment of bronchial allergies.

The preparation of pyrido[2,3-d]pyrimidines containing a 5-carboxy or 5-carbalkoxy group is described by Fatutta, Cazz. Chom. Ital. 93, (5), 576-84 (1963); C.A. 59, 6401 (1963). No utility is reported for the products. Mulvey et al., J. Org. Chem. 29, 2903-7 (1964) report the synthesis of pyrido[2,3-d]pyrimidines having a 6-carboxy, 6-carboxamido, or 6-carbalkoxy group. No reports of pyrido[2,3-d]pyrimidin-4(3H)-one-2-carboxylic acids, esters, or amides of formula III below appear in the literature. The preparation of 2-methylpyrido[2,3-d]pyrimidin-4(3H)-one and its use as a saluretic and circulatory stimulating agent is described in South African Patent 6902561, granted October 21, 1969 (C.A. 72, 125071s, 1970).

Allergic reactions, the symptoms resulting from an antigen antibody interaction, manifest themselves in a wide variety of ways and in diffusely different organs and tissues. One of the most disabling and debilitating of the allergic reactions is asthma, a functional condition of the bronchi characterized by periodic and spasmodic attacks of breathlessness, wheezing, coughing, and expectoration of mucous.

Efforts to discover medicinal agents to alleviate the symptoms of the abnormal physiologic state have been extensive. As early as 1910, Metthews, Brit. Med. J., 1, 441 (1910) reported the bronchodilator effects of epinephrine. Since then, Chen and Schmidt, J. Pharmacol. Exper. Therap., 24, 339 (1924) reported the use of the alkaloid ephedrine as an orally efficacious bronchodilator with the same spectrum of activity as epinephrine. In 1940, Konzett, Arch. Exp. Path. Pharmak. 197, 27 (1940) outlined the effects of the potent aerosol bronchodilator isoproterenol. Cullum et al., Brit. J. Pharmacol. Exp. 35, 141 (1969) reported the pharmacology of solbutamol, a potent bronchodilator of prolonged duration, and active via both oral and aerosol administration. Many bronchodilator preparations contain theopylline. These are generally less potent than the sympathomimetic amines such as isoproterenol and solbutamol, and are ineffective in aerosol administration.

Recently, Cox and co-workers, Adv. in Drug Res., 5, 115 (1970) described the pharmacology of disodium cromoglycate [1,3-bis-(2-carboxychromon-5-yloxy)-2-hydroxypropane, Intal], an agent useful in the treatment of bronchial asthma. It is not a bronchodilator but mediates its therapeutic effects by a unique mechanism of action. It suffers from the lack of oral efficacy and, for optimum results, is administered by inhalation as a solid inhalant.

Although the aforementioned agents represent outstanding contributions toward the treatment of asthma, many of them exert the undesired side effect of cardiac stimulation.

SUMMARY OF THE INVENTION

It has now been found that fused pyrimidines having the formulae ##SPC1##

and the pharmaceutically-acceptable cationic salts thereof expert potent allergy effects in mammals, including man, by an Intal-like mechanism. In contrast to Intal, many of these compounds are effective via intraperitioneal and oral administration, as well as by inhalation and intravenous administration.

In the above formulae:

R is selected from the group consisting of methyl, ethyl, acetyl, and COR^(o) wherein R^(o) is selected from the group consisting of hydroxy, alkoxy, hydroxyalkoxy, amino, hydroxyamino;

Y is selected from the group consisting of (a) hydrogen and (b) alkyl, carbalkoxyalkyl, carboxyalkyl, -(CH₂)_(m) -O-CO-C₆ H₅ and (CH₂)_(m) -O-CO-alkyl; with the proviso that R^(o) is amino or hydroxyamino, Y is hydrogen;

m is an integer from 2 up to, and including, 4;

R₁ is selected from the group consisting of hydrogen, alkyl and phenyl;

each of R₂, R₃, R₄, and R₅ is selected from the group consisting of hydrogen, alkyl, alkoxy, halo, benzyloxy, hydroxy, thiol, alkanoyloxy, benzyloxy, methylthio, methylsulfinyl, benzylthio and benzylsulfinyl;

R₂ and R₃, R₃ and R₄ when taken together are alkylenedioxy and are selected from the group consisting of methylenedioxy and ethylenedioxy.

The terms "alkyl," "alkanoyloxy" and "alkoxy" as used herein include alkyl, alkanoyloxy and alkoxy groups of from 1 up to, and including, 4 carbon atoms; the term "hydroxyalkoxy" includes hydroxyalkoxy groups having from 2 up to, and including, 4 carbon atoms; and the term "carbalkoxy" includes carbalkoxy groups having from 2 up to, and including, 5 carbon atoms. The term "halo" includes chloro, bromo, fluoro, and iodo.

Compounds of the above formulae are, except for those of formula I wherein Y is hydrogen, R is methyl and each of R₁ -R₅ is hydrogen and those of formula III wherein R is methyl and each of R₂ -R₄ is hydrogen, new compounds.

By the term "pharmaceutically-acceptable cationic salts" is intended salts such as the alkali metal salts, e.g., sodium and potassium; alkaline earth metal salts such as calcium and magnesium; aluminum salts; ammonium salts; and salts with organic bases, e.g., amines such as triethylamine, tri-n-butylamine, piperidine, triethanolamine, diethylaminoethylamine, N,N'-dibenzylethylenediamine and pyrrolidine.

The following compounds are of particular interest to this invention:

Formula I: R is CH₃ or COR^(o) wherein R^(o) is alkoxy or hydroxy; each R₁, R₂, and R₅ is hydrogen; and R₃ and R₄ are hydrogen, hydroxy, or alkoxy; provided that when one of R₃ or R₄ is hydroxy, the other is alkoxy.

Formula II: R is COR" wherein R" is hydroxy; R₂ is hydrogen; and each of R₃ and R₄ is alike and is hydrogen or alkoxy.

Formula III: R is COR^(o) wherein R^(o) is hydroxy or alkoxy; R₂ is hydrogen; and each of R₃ and R₄ is alike and is hydrogen or alkoxy.

Special interest exists in the following compounds of formula I:R R₁ R₂ R₃ R₄ R₅______________________________________COR^(o) ; H H OCH₃ OCH₃ HR^(o) = OH, H H OH OCH₃ Halkoxy H H OCH₃ OH H______________________________________

Compounds of formulae I, II, and III wherein R is carbalkoxy are also valuable intermediates for production of compounds wherein R is carboxy or carbamyl. This is especially true of formula II compounds since those wherein R is carbalkoxy appear of marginal interest as antiallergy agents.

Compounds wherein any of R₂ -R₅ is benzyloxy, benzylthio or methylthio, serve as intermediates for compounds wherein the R group is hydroxy, alkanoyloxy thiol, methylsulfinyl, or benzylsulfinyl.

The antiallergy property of the compounds of this invention is evaluated by the passive cutaneous anaphylaxis (PCA) test (Ovary, J. Immun. 81, 355, 1958). In the PCA test, normal animals are injected intradermally (i.d.) with antibodies contained in serum obtained from actively sensitized animals. The animals are then challenged intravenously with antigen mixed with a dye such as Evans' Blue. The increased capillary permeability caused by the antigen-antibody reaction causes the dye to leak from the site of the antibody injection. The test animals are then asphyxiated and the intensity of the reaction determined by measuring the diameter and intensity of the blue coloration on the inner surface of the animals skin.

DETAILED DESCRIPTION OF THE INVENTION

The compounds of this invention can be prepared by a variety of methods. Since structures I, II and III have in common the fused pyrimidine ring, the same reactions can be used to complete this portion of each structure. Similarly, the fused pyridine rings of structures I and III can be obtained by the same type of reaction using, of course, the appropriate precursor in each instance.

Compounds of formula (R = COR^(o)) can be prepared by methods in which use is made of the intact carbocyclic ring and the quinoline and pyrimidine systems built up in many ways. These methods have, as a common ground, the construction of the 2-aminoquinoline-3-carboxamide, or 3-carboxy acid or ester thereof, from the intact carbocyclic ring and subsequent use of the quinoline system as a basis for building up the pyrimidine ring. The general scope of these methods is summarized in the sequence below wherein R' represents hydroxy, alkoxy and amine: ##SPC2##

The preferred process on the basis of yield and quality of the final product of formula I is illustrated by Route A. Alternate methods, e.g., Route B, can also be used as is discussed below.

In each of Routes A and B, the condensation involves the carbocyclic aldehyde or ketone with an active methylene nucleophile. The requisite carbocyclic aldehydes or ketone reactants are known materials or are prepared by methods described in the art. By "active methylene" nucleophile is meant a compound having a relatively acidic methylene group; that is, a methylene group linked to one, and preferably two, electron-withdrawing groups such as --CN, --COCl, --C(NH)NH₂, COR', --C(NH)-O-alkyl and -CO-alkyl.

The condensation is conducted in the presence of a reaction-inert solvent; that is, a solvent which is not changed as a result of the reaction even though it may participate in the reaction in the role of a catalyst or in salt formation with a reactant or product.

Suitable solvents are alkanols, such as methanol, ethanol, isopropanol, n-butanol and n-hexanol; chlorinated solvents such as methylene chloride, ethylene chloride, chloroform and carbon tetrachloride; pyridine; aromatic hydrocarbons such as benzene, toluene, xylene; hexane; and N,N'-dimethylformamide. Other solvents are found by simple experimentation. Methanol is a preferred solvent, especially when using piperidine as catalyst, because, of the satisfactory yields, ease of separation and purity of products. A solvent system of piperidine and pyridine is frequently a useful system.

A catalyst is often used to facilitate the condensation even when the nucleophiles possess two activating groups as do derivatives of cyanoacetic acid. Suitable catalysts are ammonia, primary, secondary, and tertiary amines, such as n-butylamine, diethylamine, triethylamine, pyridine, piperidine, pyrrolidine, alkali metal alkoxides and fluorides, stannous fluoride, and basic ion-exchange resins of the amine type, e.g., Amberlite IR-45 (a weakly basic polystyrene with polyamine groups, available from Rohm & Haas Co.) and De-Acidite G (polystyrene resin with diethylamino groups; available from the Permutit Co., Ltd., London).

The amount of catalyst used is not critical but can vary over a wide range, i.e., from about 0.1% to about 100% by weight based upon the carbocyclic aldehyde reactant. The favored range of catalyst is from about 10% to about 30% by weight of the carbocyclic aldehyde reactant.

The reaction is conducted at a temperature of from about 0° C. to about 50° C. and generally at about ambient temperature for periods of from about one-fourth to five hours. The products generally separate from the reaction mixtures as solids and are recovered by filtration. Those which do not separate as solids are recovered of the solvent or by pouring into a large volume of a non-solvent for the product.

The use of an o-nitrobenzaldehyde or an R₁ -(2-nitrophenyl) ketone as the reactant (Route A) produces an α-cyano-β-(2-nitrophenyl)acrylamide derivative, e.g., an amide when R' of the active methylene reactant is NH₂, which must subsequently be reduced and cyclized to provide the desired 2-aminoquinoline-3-carboxylic acid derivative. Reduction (of the nitro group to an amino group) is accomplished by a variety of reagents. In brief, any reagent which will selectively or preferentially reduce the nitro group to an amino group can be used. Representative of such reagents are metal-acid combinations such as iron-acetic acid, iron-hydrochloric acid, tin or stannous chloride-hydrochloric acid, zinc-hydrochloric acid, zinc dust-alkali; and catalytic hydrogenation using catalysts such as platinum, palladium and Raney nickel.

The reduced product appears to cyclize immediately, or almost immediately, to provide the 2-aminoquinoline-3-carboxylic acid derivative.

When using an o-aminobenzaldehyde as reactant (Route B) the condensation product with the cyanoacetic acid derivative cyclizes at a very rapid rate to the 2-aminoquinoline-3-carboxylic acid derivative as noted above for the reaction product of Route A.

Formation of the fused pyrimidine ring with its 2-carboxylic acid derivative (ester or amide) substituent can be accomplished by a number of methods. For convenience, these methods are considered on the basis of the structure of reactant W-Z which contributes the one-carbon fragment to complete the pyrimidine ring system upon the appropriate 2-aminoquinoline-3-carboxylic acid derivative;

1. Reaction of a 2-aminoquinoline-3-carboxamide (formula V, R'=NH₂) with:

a. a dialkyl oxalate;

b. a monoacid halide (chloride, bromide) of a half-alkyl oxalic acid ster;

c. an alkyl cyanoformate;

d. a dialkyl ester of monoiminooxalic acid (a carbalkoxy formimidate);

e. an alkyl ester of oxamidic acid;

f. 1-cyanoformamide;

g. cyanogen; and

h. 1-carbalkoxyformamidine; or

2. Reaction of an alkyl 2-aminoquinoline-3-carboxylate with:

a. a 1-carbalkoxyformamidine.

Reactant W-Z (an oxalic acid derivative) of the step common to Routes A and B provides the one-carbon fragment needed to complete the fused pyrimidine ring. It may, depending upon choice of reactants, also provide the --NH group. It represents cyanogen and W-COR^(o) wherein W is --COCl, -CN, -COR^(o), -CO-alkyl, -C(NH)NH₂ and -C(NH)O-alkyl. When W-Z is an alkyl ester of oxamidic acid, e.g., H₅ C₂ OOC-CONH₂, the cyclization reaction occurs so as to produce the amide (R=CONH₂) of formula I.

It is evident that one of the two reactants in the final step of Routes A and B above must provide the --NH moiety. When the reactant upon which the fused pyrimidine ring is to be formed contains a carboxamide group (e.g., formula V wherein --COR' is -CONH₂), reactant W-Z can be any of the substances enumerated above; i.e., cyanogen or W-COR^(o). However, when the reactant upon which the fused pyrimidine ring is to be formed does not contain a carboxamide group; that is, when -COR' is carboxy or carbalkoxy, or haloformyl, reactant W-Z must provide the --NH group.

The favored process comprises using the appropriate 2-amino-3-carboxamide (formula V wherein R'=NH₂) as reactant which is condensed with the W-Z reactant to provide only the one-carbon fragment to complete the pyrimidine ring.

Reaction of the 2-aminoquinoline-3-carboxylic acid derivative with reactant W-Z is carried out in a reaction-inert solvent and desirably, when W is -COR^(o) or C(NH)-O-alkyl, one which will permit removal of by-product alcohol and water by distillation. Representative solvents for this cyclization are aromatic hydrocarbons such as xylene, toluene, benzene; an excess of the chosen dialkyloxalate reactant; tetralin and decalin.

Suitable solvents are readily determined by experimentation. A favored solvent when W-Z is a dialkyl oxalate is an excess of the dialkyl oxalate because of its ability to solubilize the reactants and to permit simple removal of by-product alcohol and water. Although the reaction temperature is not critical, the reaction is generally run at an elevated temperature to facilitate removal of alcohol and water. Temperatures of from about 150° C. to about 185° C. are useful when a dialkyl oxalate is used as solvent. Lower temperatures can be employed with the more reactive oxalic acid derivatives (W-Z) such as ethyl oxalylchloride. A final fusion or heating period is sometimes beneficial to achieving maximum cyclization and yield of the desired pyrimido[4,5-b]quinolin-4(3H)-one-2-carboxylic acid derivative. The addition of a small amount of a base, such as sodium hydride and alkali metal alkoxides, is frequently useful in promoting cyclization, expecially in making compounds of formula II.

When reactant W-Z results in production of the 2-cyano derivative rather than a 2-COR^(o) derivative, the cyano group is converted to the corresponding 2-carboxamide by acid hydrolysis. When W-Z is a monoacid halide of a half-alkyl oxalic acid ester, an acid acceptor; i.e., an organic or inorganic base, such as triethylamine, pyridine, sodium methoxide, sodium hydroxide, is used to neutralize the by-product acid formed.

When reactant W-Z represents an alkyl ester of glycolic acid, the fused pyrimidines of formulae I-III produced bear the hydroxymethyl group at the 2-position. Such compounds serve as precursors for corresponding aldehydes. The conversion is accomplished by oxidation using chromic anhydride in pyridine (Sarett reagent) or chromic anhydride-pyridine-water (Cornforth reagent). The latter reagent is preferred because of its ease of preparation and handling relative to the Sarett reagent. Further oxidation of the 2-carboxaldehyde by, for example, chromic anhydride-sulfuric acid at about 30°C-50°C. produces the 2-carboxylic acid.

Alternatively, the appropriate alkyl benzyloxy acetate; e.g., ethyl benzyloxyacetate, is used as reagent W-Z to produce compounds of formulae I-III wherein the substituent is a 2-benzyloxymethyl group. The benzyl groups of the 2-benzyloxymethyl derivatives thus-produced are removed by reaction with strong acids such as trifluoroacetic acid in the manner described below.

The 5-substituted compounds in formula I are also prepared from the appropriate 4-substituted 2-aminoquinoline-3-carboxamide which, in turn, is prepared by the reaction of malononitrile with the appropriate R₁ -(2-aminophenyl) ketone, e.g., 2-aminobenzophenone (R₁ -phenyl) and aminoacetophenone (R₁ =CH₃). The 2-amino-3-cyano-4-substituted quinoline thus produced is hydrolyzed to the corresponding 4-substituted-2-aminoquinoline-3-carboxamide by heating with 95% sulfuric acid, followed by aqueous work-up. The process is that described by Campaigne et al., J. Hetero. Chem. 8, 111-120 (1971).

A further method comprises condensation of an appropriate o-nitrobenzaldehyde with a dialkylmalonate to produce an alkyl α-carbalkoxy-β-(2-nitrophenyl)acrylate which is subsequently reduced to the corresponding alkyl α-carbalkoxy-β-(2-aminophenyl)acrylate according to procedures described above. The reduced product spontaneously cyclizes to an alkyl 2-oxo-quinoline-3-carboxylate. The 2-oxo derivative is heated directly with 1-carbalkoxy formamidine in ethanol using sodium ethoxide to give compounds of formula I. Or, the 2-oxo derivative is chlorinated with a suitable chlorinating agent such as phosphorous trichloride, phosphorous pentachloride, phosphorous oxychloride in a reaction-inert solvent such as an aromatic hydrocarbon or a halogenated aliphatic or aromatic hydrocarbon (benzene, toluene, xylene, methylene chloride, ethylene chloride, chloroform, chlorobenzene) at a temperature of from about 50° C. to 100° C.

The alkyl 2-chloroquinoline-3-carboxylate thus produced is reacted with an alkyl 1-guanylformate to provide the corresponding alkyl pyrimido[4,5-b]quinolin-4(3H)-one-2-carboxylate. This cyclization is carried out according to known methods.

Still a further method comprises construction of the central pyridine ring. It involves condensation of the appropriate o-nitrobenzaldehyde with an ester, e.g., an alkyl ester of 4,6-dioxo(3H,5H)-tetrahydropyrimidine-2-carboxylic acid, in the presence of a base to produce the corresponding 4,6-dioxo(3H,5H)-5-(2-nitrobenzylidene)tetrahydropyrimidine-2-carboxylic acid ester which, upon subsequent reduction in the manner described above, spontaneously cyclizes to a compound of formula I. A variation on this method involves condensation of the o-nitrobenzaldehyde malondiamide, followed by reaction of the product with a dialkyl oxalate to form the above nitrobenzylidene compound.

Compounds of formula II (R = COR^(o)) are prepared by methods similar to those described above for compounds of formula I but using, of course, in place of a 2-aminoquinoline-3-carboxamide, a 2-aminonaphthalene-3-carboxamide. The reaction conditions for achieving construction of the pyrimidine ring are substantially the same as those described for compounds of formula I.

The compounds of formula III (R = COR^(o)) are similarly prepared from appropriate 2-aminonicotinamide or precursors thereto, e.g., 2-aminonicotinic acid, 2-nitropyridine-3-carboxamides or 3-carboxylic acids, 2-nitro- or 2-aminopyridine-3-carboxaldehydes. In preparing compounds of formulae II and III, the favored routes comprise reacting W-Z with the appropriate 2-amino-3-carboxamide reactant.

The amide and hydroxamic acid derivatives of formula I, II and III (R^(o) =NH₂, NHOH) are prepared by methods described above from W-CONH₂, W-CONHOH or by reaction of the precursor alkyl ester of formulae I, II, and III with ammonia or hydroxylamine. The usual procedure when comprises reacting the appropriate ester with ammonia or with hydroxylamine hydrochloride, usually in excess, in the presence of an acid acceptor such as triethylamine. The reaction is facilitated by heating under pressure, i.e., the reaction is carried out in a bomb, in a solvent such as ethanol for from about 4 to about 20 hours and the product then recovered by suitable means.

The 2-methyl and 2-ethyl analogs of formulae I and III are prepared by cyclization of the appropriate 2-amino-quinoline-3-carboxamide or 2-aminonicotinamide with the appropriate alkanoic acid anhydride e.g., acetic or propionic anhydride; or with triethyl orthoacetate or triethyl orthopropionate in the presence of sulfuric acid. Treatment of the product with dilute alkali followed by reacidification provides the 2-methyl or ethyl analogs. The preparation of 2-methylpyrimido[4,5-b]quinolin-4(3H)-one by this procedure is reported by Taylor et al., J. Am. Chem. Soc. 78, 5108-15 (1956) as previously noted. The 2-ethyl analogs, new compounds, are primarily of value as intermediates for preparation of the corresponding 2-acetyl derivatives of formulae I and III.

The 2-acetyl derivatives of formulae I and III are prepared by oxidation of the corresponding 2-ethyl derivatives with selenium dioxide and water in a suitable solvent medium, for example, dioxane. In the usual procedure the 2-ethyl derivative and selenium dioxide are used in about a 2:1 molar ratio at an elevated temperature, e.g. from about 50° to about 100°C. Additional selenium dioxide beyond the 2:1 molar ratio can be used to expedite the oxidation.

The 2-methyl analogs of formulae I and III are useful intermediates for preparation of corresponding 2-carboxaldehydes and 2-carboxylic acids, and from them, of corresponding esters of the acids.

A useful procedure for preparing 2-carboxylic acids comprises refluxing the appropriate 2-methyl derivative in a solvent such as ethanol with a slight excess of benzaldehyde in the presence of sodium ethoxide or piperidine, or other base, to form the benzylidine adduct. The amount of base used is not critical but can vary from a catalytic amount (<1%) up to an equivalent amount. The benzylidene adduct is isolated by removal of the solvent and is then oxidized to the acid by excess cold potassium permanganate in aqueous acetone, or by ozonolysis. Alternatively, the methyl group is converted to the corresponding 2-bromomethyl compound by reaction with N-bromosuccinimide in chloroform. The succinimide by-product is removed by concentration of the reaction mixture and filtration. The filtrate is taken to dryness and the residue dissolved in aqueous sulfuric acid and treated with chromium trioxide to provide the acid.

Conversion of the 2-methyl groups to 2-carboxaldehyde groups is achieved by oxidation with selenium dioxide and water in the manner described above for conversion of 2-ethyl to 2-acetyl groups. Alternatively, the 2-methyl group is transformed to the 2-bromomethyl group according to the procedure mentioned above which is then hydrolyzed to the corresponding 2-hydroxymethyl group.

The alkylene glycol esters of formula I-III (R=COR^(o) wherein R^(o) is hydroxyalkoxy) are conveniently prepared by a base catalyzed transesterification process. The process comprises treating a compound of formulae I-III wherein R^(o) is alkoxy with an alkylene glycol, preferably in the presence of a catalytic amount of a base (i.e., from about 5% to about 20% by weight based upon the alkylene glycol used), such as triethylamine or calcium hydroxide, in air at a temperature of from about 20°C. to about 50°C. Higher temperatures can be used but appear to offer no advantages.

Compounds of formula I wherein Y is other than hydrogen are prepared by alkylation of formula I compounds wherein Y is hydrogen. The procedure comprises formation of the sodium salt by reaction of the appropriate formula I compound with sodium hydride in a suitable solvent, e.g., N,N-dimethylformamide. Reaction of the sodium salt with I-Y, Br-Y (or Cl-Y) affords the alkylated derivative. When using Cl-Y as alkylating agent, the presence of a small amount of sodium or potassium iodide (from about 10% to 20% by weight of Cl-Y) serves to accelerate the reaction.

Compounds of formulae I-III wherein any of R₂, R₃, R₄, or R₅ is benzyl methylsulfinyl are readily prepared from the corresponding thioether compounds by oxidation with an appropriate oxidizing agent such as hydrogen peroxide or a per acid such as m-chlorperbenzoic acid accord to methods known to those skilled in the art.

The methylthio compounds are, in turn, readily prepared by the reaction of the corresponding chloro compound of formula IV; i.e., an α-cyano-β-(2-nitro-chlorophenyl)acrylamide, with sodium methyl mercaptide. Modifications of this method are obvious to those skilled in the art. For example, the methylthio ether of formula IV can be made by in situ formation of the methyl mercaptide salt. Similarly, benzyloxy, benzylthio, and alkoxy compounds are prepared by reacting the corresponding chloro compound of formula IV with sodium benzyloxide, sodium benzylmercaptide, or a sodium alkoxide.

Compounds of formulae I-III wherein any of R₂ -R₅ is benzyloxy or benzylthio serve as intermediates for the corresponding hydroxy and thiol compounds and acyl derivatives thereof. Debenzylation is conveniently accomplished by treating the benzyl ether or benzylthio ether with trifluoroacetic acid. The debenzylated products are obtained as their trifluoroacetate salts. The hydroxy and thiol compounds, in turn, are intermediates for preparation of corresponding alkanoyloxy, and benzoyloxy derivatives by acylation using the appropriate acid anhydride e.g. acetic anhydride. A catalytic amount of p-toluenesulfonic acid is generally used to expedite the reaction.

The products of this invention and the pharmaceutically-acceptable cationic salts thereof, are useful for the control (propylactic and therapeutic treatment) of allergic symptoms and reactions in mammals, and can be administered either as individual therapeutic agents or as mixtures of therapeutic agents, for example, with theophylline or sympathomimetic amines. They can be administered alone, but are generally administered with a pharmaceutical carrier selected on the basis of the chosen route of administration and standard pharmaceutical practice. For example, they can be combined with various pharmaceutically-acceptable inert carriers in the form of tablets, capsules, lozenges, troches, hard candies, powders, aerosol sprays, aqueous suspensions or solutions, injectable solutions, elixirs, syrups and the like. Such carriers include solid diluents or fillers, sterile aqueous media and various non-toxic organic solvents. Moreover, the oral pharmaceutical compositions of this invention can be suitably sweetened and flavored by means of various agents of the type commonly used for this purpose.

The particular carrier selected and the proportion of active ingredient to carrier are influenced by the solubility and chemical nature of the therapeutic compounds, the chosen route of administration and the needs of standard pharmaceutical practice. For example, when the compounds of this invention are administered orally in tablet form, excipients such as lactose, sodium citrate, calcium carbonate and dicalcium phosphate can be used. Various disintegrants such as starch, alginic acids and certain complex silicates, together with lubricating agents such as magnesium stearate, sodium lauryl sulphate and talc, can also be used in producing tablets for the oral administration of these compounds. For oral administration in capsule form, lactose and high molecular weight polyethylene glycols are among the preferred materials for use as pharmaceutically-acceptable carriers. Where aqueous suspensions are to be used for oral administration, the compounds of this invention can be combined with emulsifying or suspending agents. Diluents such as ethanol, propylene glycol, glycerine and chloroform and their combinations can be employed as well as other materials.

For the purpose of parenteral administration and inhalation, solutions or suspensions of these compounds in sesame or peanut oil or in aqueous propylene glycol solutions can be employed, as well as sterile aqueous solutions of the soluble pharmaceutically-acceptable salts described herein. These particular solutions are especially suited for intramuscular and subcutaneous injection purposes should such method of administration be desired. The aqueous solutions, including those of the salts dissolved in pure distilled water, are also useful for intravenous injection purposes provided that their pH is properly adjusted beforehand. Such solutions should also be suitably buffered, if necessary, and the liquid diluent first rendered isotonic with sufficient saline or glucose.

The compounds can be administered to asthmatic subjects suffering from bronchoconstriction by means of inhalators or other devices which permit the active compounds to come into direct contact with the constricted areas of the tissues of the subject.

When administered by inhalation, the compositions can comprise (1) a solution or suspension of the active ingredient in a liquid medium of the type mentioned above for administration via a nebulizer; (2) a suspension or solution of the active ingredient in a liquid propellant such as dichlorodifluoromethane or chlorotrifluoroethane for administration from a pressurized container; or (3) a mixture of the active ingredient and a solid diluent (e.g., lactose) for administration from a powder inhalation device. Compositions suitable for inhalation by means of a conventional nebulizer will comprise about 0.1 to about 1% of active ingredient; and those for use in pressurized containers will comprise from about 0.5 to about 2% of active ingredient. Compositions for use as powder inhalants can comprise ratios of active ingredient to diluent of from about 1:0.5 to about 1:1.5.

It is necessary that the active ingredient form a proportion of the composition such that a suitable dosage form will be obtained. Obviously, several dosage unit forms can be administered at about the same time. Although compositions with less than 0.005% by weight of active ingredient might be used in certain instances, it is preferred to use compositions containing not less than 0.005% of the active ingredient; otherwise, the amount of carrier becomes excessively large. Activity increases with the concentration of the active ingredient. The composition may contain 10, 50, 75, 95 or an even higher percentage by weight of the active ingredient.

The PCA reaction test procedure employed to evaluate the compounds of the present invention demonstrates an excellent correlation between activity for compounds in this test and their utility in the treatment of allergic asthma. The ability of agents to interfere with PCA reactions is measured in male Charles River Wistar rats, 170-210 g. Reaginic antisera is prepared according to Mota, Immunology, 7, 681 (1964) using hen egg albumin and B. pertussis. Hyperimmune antisera to hen egg albumin is prepared according to Orange, et al., J. Exptl. Med., 127, 767 (1968). 48 hours prior to antigen challenge the reaginic antisera is injected intradermally (i.d.) into the shaved skin of a normal rat's back; five hours before challenge the hyperimmune antisera is similarly injected; five hours later, at a third site, 60 mcg. histamine dihydrochloride is injected i.d. as a check for antihistaminic and unspecific types of blockage; the compounds of the instant invention or saline are then administered i.v. and immediately followed by 2.5 mg. Evan's blue dye and 5 mg. egg albumin in saline. In the case of oral administration Evan's blue dye and egg albumen are given five minutes after administration of the drug. Thirty minutes later the animals are asphyxiated using chloroform and the skin of the back removed and reversed for observation. A score is assigned each injection site equal to the product of the diameter of the site in mm. and a grade of 0.1, 0.5, 1, 2, 3 or 4 proportional to intensity of dye coloration. The scores for a given injection site are summed for each group of 8 animals and compared to the saline treated controls. The difference is expressed as percent blockade due to the compound employed.

Compounds representative of those in the present invention are tested by the aforementioned procedure, and the resulting activities are reported as the degree (%) of protection. Disodium cromoglycate, a commerial antiallergy agent, is included for comparison. ##STR1##

                                      TABLE I                                      __________________________________________________________________________     Antiallergy Activity of Pyrimido[4,5-b]                                        Quinolin-4(3H)-One-2-Carboxylic Acid                                           Derivatives (Formula I)                                                                                         I.V.*       Oral                              R        R.sub.1                                                                            R.sub.2                                                                            R.sub.3                                                                             R.sub.4                                                                               R.sub.5                                                                            mg/kg  %    mg/kg                                                                                %                           __________________________________________________________________________     COOC.sub.2 H.sub.5                                                                      H   H   H    H      H   10    100   100   80                                                           3      99   60   100.sup.3                                                     1     100.sup.4                                                                            30    72.sup.3                                                     0.3    82.sup.6                                                                            10    75.sup.3                                                     0.1    45.sup.6                                                                            3     69.sup.2                                                     0.03   30.sup.6                                                                            1     12.sup.2                    COONa    H   H   H    H      H   1      92   100   96                                                           0.3    68   30    57                                                                       10    0                           COO(n-C.sub.4 H.sub.9)                                                                  H   H   H    H      H   1      90   30    50                                                           0.03   6                                      CONH.sub.2                                                                              H   H   H    H      H   3      98   60    23                          COOC.sub.2 H.sub.5                                                                      H   H   OCH.sub.3                                                                           OCH.sub.3                                                                             H   1     100   10    80.sup.17                                                    0.3    90.sup.3                                                                            3     75.sup.22                                                    0.1    90.sup.12                                                                           1     51.sup.26                                                    0.03   75.sup.14                                                                           0.03  30.sup.20                                                    0.01   70.sup.17                                                               0.003  36.sup.14                              COO(n-C.sub.4 H.sub.9)                                                                  H   H   OCH.sub.3                                                                           OCH.sub.3                                                                             H   3      90   30    81                                                           0.3    94                                                                      0.03  100                                                                      0.003  64                                     COOCH.sub.2 CH.sub.2 OH                                                                 H   H   OCH.sub.3                                                                           OCH.sub.3                                                                             H   3      97   10    65                                                           0.3    94                                                                      0.03   92                                     CONH.sub.2                                                                              H   H   OCH.sub.3                                                                           OCH.sub.3                                                                             H   3      87   30    4                           CONHOH   H   H   OCH.sub.3                                                                           OCH.sub.3                                                                             H   3      92   60    3                           COONa    H   H   OCH.sub.3                                                                           OCH.sub.3                                                                             H   0.1   100   10    17.sup.2                                                     0.03   96                                                                      0.01   71                                     CH.sub.3 H   H   OCH.sub.3                                                                           OCH.sub.3                                                                             H   3      77.sup.4                                                                            3     67.sup.6                                                     0.3    45.sup.4                                                                            1     43.sup.6                                                     0.03   11.sup.4                               C.sub.2 H.sub.5                                                                         H   H   OCH.sub.3                                                                           OCH.sub.3                                                                             H   3      56   60    0                           COCH.sub.3                                                                              H   H   OCH.sub.3                                                                           OCH.sub.3                                                                             H   3     100   10    15                                                           1      21                                                                      0.1    19.sup.2                               COOC.sub.2 H.sub.5                                                                      H   H   OCH.sub.3                                                                           OCH.sub.3 H.sub.5                                                                     H   3      97   30    80.sup.4                                                     0.3    94.sup.2                                                                            10    73.sup.7                                                     0.1    94.sup.2                                                                            3     52.sup.6                                                     0.03   85.sup.4                                                                            1     44.sup.7                                                     0.01   57.sup.3                                                                            0.3   31.sup.2                                                     0.003  66.sup.3                               COOC.sub.2 H.sub. 5                                                                     H   H   --O--CH.sub.2 --O--                                                                        H   1      70   30    0                                                            0.1    0                                      COOC.sub.2 H.sub.5                                                                      H   H   --O--CH.sub.2 CH.sub.2 --O--                                                               H   3      72                                                                      1      64                                     COOC.sub.2 H.sub.5                                                                      H   H   OC.sub.2 H.sub.5                                                                    OC.sub.2 H.sub.5                                                                      H   3     100   10       81.sup.3                                                  0.1    90.sup.2                                                                            3        64.sup.6                                                  0.03   60.sup.3                                                                            1        40.sup.6                                                  0.01   43.sup.3                                                                            0.3      42.sup.4                                                  0.003  21.sup.3                               COOC.sub.2 H.sub.5                                                                      H   H   OC.sub.2 H.sub.5                                                                    OCH.sub.3                                                                             H   3     100   10       92.sup.4                                                  0.1    99.sup.2                                                                            3        83.sup.6                                                  0.03   85.sup.4                                                                            1        76.sup.7                                                  0.01   72.sup.4                                                                            0.3      56.sup.5                                                  0.003  50.sup.4                               COOC.sub.2 H.sub.5                                                                      H   H   OC.sub.2 H.sub.5                                                                    O--(n-C.sub.4 H.sub.9)                                                                H   3      90   10       67.sup.3                                                  1      95   3        43.sup.2                                                  0.3    44.sup.3                                                                            1        3.sup.2                                                   0.1    28                                                                      0.03   0                                      COOC.sub.2 H.sub.5                                                                      H   H   OC.sub.2 H.sub.5                                                                    OC.sub.7 H.sub.7                                                                      H   3      71.sup.4                                                                            30       0                                                         0.3    30.sup.2                                                                            10       0                                                         0.1    3.sup.2                                COOC.sub.2 H.sub.5                                                                      H   H   H    OCH.sub.3                                                                             OCH.sub.3                                                                          3     100   60       26                                                        0.3    39   10       0                        COOC.sub.2 H.sub.5                                                                      H   H   OCH.sub.3                                                                           H      H   3     100   10       43                                                        0.3    87   3        32                                                        0.1    68.sup.2                               COOC.sub.2 H.sub.5                                                                      H   H   H    OCH.sub.3                                                                             H   3       90  10       43                                                        0.3    20                                                                      0.03   0                                      COOC.sub.2 H.sub.5                                                                      H   OCH.sub.3                                                                          H    H      OCH.sub.3                                                                          3     100   30       23                                                        0.3    25                                                                      0.03   0                                      COOC.sub.2 H.sub.5                                                                      H   H   OCH.sub.3                                                                           H      OCH.sub.3                                                                          3      97   30       41                                                        0.3    38                                                                      0.03   14                                     COOC.sub.2 H.sub.5                                                                      H   H   SCH.sub.3                                                                           H      H   3      88   30      100                                                        0.3    91   10       66                                                        0.03   8    3        21                                                                    1        0                        COOC.sub.2 H.sub.5                                                                      H   H   SOCH.sub.3                                                                          H      H   3     100   30       12                                                        0.3    58                                                                      0.03   0                                      COOC.sub.2 H.sub.5                                                                      H   H   OCH.sub.3                                                                           OCH.sub.3                                                                             OCH.sub.3                                                                          3      88   10       0                                                         0.3     23                                                                     0.03   33                                     COOC.sub.2 H.sub.5                                                                      H   H   H    F      H   0.1    2                                      COOC.sub.2 H.sub.5                                                                      H   H   F    H      H   3      82   30       17                                                        1     90                                                                       0.03   0                                      COOC.sub.2 H.sub.5                                                                      H   H   Cl   H      H   1      70                                                                      0.03   0                                      COOC.sub.2 H.sub.5                                                                      H   Cl  H    H      H   1      96   30       57                                                        0.03   11                                     COOC.sub.2 H.sub.5                                                                      H   H   H    H      3   100   10     0                                                                 1      95   1        0                                                         0.3    51                                                                      0.1    9                                      COOC.sub.2 H.sub.5                                                                      H   H   CH.sub.3 COO                                                                        OCH.sub.3                                                                             H   3     100   10       40                                                        .003   53                                     COOC.sub.2 H.sub.5                                                                      H   H   OH   OCH.sub.3                                                                             H   0.03   97   30       6                                                         0.01   98                                                                      0.003  94                                                                      0.0003                                                                                38                                     COOH     H   H   OH   OCH.sub.3                                                                             H   3     100                                                                      0.01   86                                                                      .003   90                                     COO-(n-C.sub.4 H.sub.9)                                                                 H   H   OH   OCH.sub.3                                                                             H   3     100   10       67.sup.2                                                  .003   52                                     COO--(n-C.sub.4 H.sub.9)                                                                H   H   OC.sub.7 H.sub.7                                                                    OCH.sub.3                                                                             H   3     100   10       12                                                        .03    57                                     COO--(n-C.sub.4 H.sub.9)                                                                H   H   CH.sub.3 COO                                                                        OCH.sub.3                                                                             H   3     100   10       36                       COOC.sub.2 H.sub.5                                                                      H   H   OCH.sub.3                                                                           OC.sub.7 H.sub.7                                                                      H   3      94                                                                      0.3    23                                     COOC.sub.2 H.sub.5                                                                      H   H   OC.sub.7 H.sub.7                                                                    H      H   3      85   10       60                                                        0.3    23   3        3                        COOC.sub.2 H.sub.5                                                                      H   H   OH   H      H   0.3    92   10       13                                                        0.03   21   3        4                        COOH     H   H   OH   H      H   3     100                                                                      0.03   91                                                                      .003   4                                      COOC.sub.2 H.sub.5                                                                      H   H   OCH.sub.3                                                                           OH     H   0.003  0                                                                       0.3   100                                                                      3.0   100                                     Disodium                         100   100   100   0                           chromoglycate                    30     99.sup.2                                                                10     89.sup.3                                                                3      78                                                                      1      56.sup.8                                                                0.3    29.sup.5                                                                0.1    19.sup.3                               __________________________________________________________________________      *The superscripts indicate a particular value is an average of two or mor      determinations.                                                          

                  TABLE II                                                         ______________________________________                                         Antiallergy Activity of Benzo[g]Quinazolin-                                    4(3H)-One-2-Carboxylic Acid Derivatives                                        (Formula II)                                                                   ______________________________________                                          ##STR2##                                                                                     I.V.      Oral                                                  R.sup.o  R.sub.2                                                                              R.sub.3                                                                              R.sub.4                                                                            R.sub.5                                                                            mg./kg.                                                                               %    mg./kg.                                                                               %                              ______________________________________                                         COOC.sub.2 H.sub.5                                                                      H     H     H   H   10     27   100    38                             COOH*    H     H     H   H   10     100  100     9                                                          3      36    30     0                                                          1      14    10    33                             ______________________________________                                          *Tested as disodium salt.                                                

                  TABLE III                                                        ______________________________________                                         Antiallergy Activity of Pyrido[2,3-d]-                                         Pyrimidin-4(3H)-One-2-Carboxylic Acid                                          Derivatives (Formula III)                                                      ______________________________________                                          ##STR3##                                                                                     I.V.      Oral                                                  R.sup.o R.sub.2                                                                               R.sub.3                                                                               R.sub.4                                                                             mg./kg.                                                                               %    mg./kg.                                                                               %                                ______________________________________                                         OC.sub.2 H.sub.5                                                                       H      H      H    10     95   100    0                                ______________________________________                                           As regards the dosage regimen of the compounds of this invention, the      physician will ultimately determine the dosage which will be most suitable      for a particular individual, and it will vary with age, weight and      response of the particular patient as well as with the nature and extent      of the symptoms, the pharmacodynamic characteristics of the particular      agent to be administered, and the route of administration chosen.      Generally, small doses will be administered initially, with a gradual      increase in the dosage until the optimum level is determined. It will      often be found that when the composition is administered orally, larger      quantities of the active ingredient will be required to produce the same      level as produced by a small quantity administered parenterally.

Having full regard for the foregoing factors, it is considered that an effective daily oral dosage of the compounds of the present invention in humans of from about 10 to about 1500 mg. per day, with a preferred range of about 10 to about 600 mg. per day in single or divided doses, or at about 0.2 to about 12 mg./kg. of body weight will effectively alleviate bronchoconstriction in human subjects. These values are illustrative and there may, of course, be individual cases where higher or lower dose ranges are merited.

When administered intravenously or by inhalation, the effective daily dose is from about 0.5 to about 400 mg. per day, and preferably from about 0.25 to 200 mg. per day, or at about 0.005 to 4 mg./kg. of body weight in single or divided doses.

Ethyl 7,8-dimethoxypyrimido[4,5-b]quinolin-4(3H)-one-2-carboxylate is active via the oral, intravenous, intraperitoneal and inhalation routes of administration. It is active intraperitoneally at 0.3-1 mg./kg.; and orally in the range of 0.3-30 mg./kg. When administered by aerosol, concentration related responses are observed using 0.3-30 mg./ml. solutions. Intravenously, it is active at a level as low as 0.003 mg./kg.

It has been observed that ethyl 7,8-dimethoxypyrimido[4,5-b]quinolin-4(3H)-one-2-carboxylate causes a marked but transient hypotension and bradycardia in dogs. The effect appears to be species specific. It appears to be relatively free of toxicity since intravenous doses of 1000 mg./kg. did not cause death in mice.

In addition to compounds of formula I above, analogs thereof wherein R₁ is substituted phenyl wherein the substituent is at least one of alkyl, alkoxy, alkylenedioxy and halo, and compounds of formulae I, II and III described herein, and analogs thereof wherein COR^(o) is an amide derived from an amino acid, e.g., ethyl glycine, and those wherein COR^(o) is replaced by a methyl or a 5-tetrazolyl group are useful antiallergy agents in the same manner as are compounds of formulae I-III. So also are analogs of formulae I, II and III, and the just described analogs thereof wherein -COR^(o) is replaced by methyl or a 5-tetrazolyl group, but in which the 4-keto group is replaced by thiono (=S).

Compounds wherein the substituent at the 4-position is thiono (=S) rather than keto (=O) are prepared in the same manner as are the keto compounds but using the appropriate cyanothionoacetic acid reactant CN-CH₂ -CSR' in place of the cyanoacetic acid reactant in either of routes A and B above. Alternatively, the appropriate 2-aminonicotinonitrile, 2-aminoquinolino-3-carbonitrile or 2-aminonaphthalene-3-carbonitrile is reacted with concentrated ammonium hydroxide at about 40°-80° C. while bubbling hydrogen sulfide through the solution for several hours, e.g., 3-5 hours; or by reacting the appropriate nitrile in pyridine containing triethylamine while bubbling hydrogen sulfide through the solution at ambient temperature. Such procedures are described by Mautner, J. Org. Chem. 23, 1450 (1958) and Bercat-Vatterone, Annales de Chimie 7, 312 (1962), respectively. The thioamides thus obtained are reacted with various oxalate derivatives Y-Z as described above for Routes A and B.

The tetrazolyl substituent (T) at the 2-position is prepared from the corresponding compound wherein the 2-substituent is cyano. The reaction comprises treating the 2-cyano compound with hydrazoic acid or, preferably, a mixture of sodium azide and ammonium chloride in N,N-dimethylformamide or dimethylsulfoxide at from about 100° C. to the reflux temperature of the mixture for from about 5 to about 24 hours. The addition of a small excess (1-5%) of ammonium azide or of a Lewis acid serves to expedite the reaction. Alternatively, a mixture of sodium azide and acetic acid can be used to generate the hydrazoic acid in situ. With this system as source of hydrazoic acid, refluxing n-butanol serves as a useful solvent and the reaction mixture is heated to reflux for about 3 to 5 days.

The required 2-cyano derivatives are obtained by reacting the appropriate 3-amino-2-naphthoic acid, 2-aminonicotinic acid or 2-aminoquinolin-3-carboxylic acid with cyanogen, as described by Griess, Ber. 2, 415 (1869) and Ber. 11, 1985 (1878). Alternatively, and preferably, the 2-cyano compounds are prepared by dehydration of the corresponding 2-carboxamides with, for example, phosphoryl chloride according to the procedure set forth in Org. Syn. Coll. Vol. 3, 535 (1955), or with phosphorous pentoxide as described by Hayoshi et al., Chem. Pharm. Bull. (Japan) 12, 43 (1964). Still further, the dehydration can be accomplished by means of p-toluenesulfonyl chloride (tosyl chloride) and pyridine according to the procedure of Stephens et al., J. Am. Chem. Soc. 77, 1701 (1955).

Additionally, 2-tetrazole derivatives of formulae I, II and III are prepared by converting the 2-carboxamide derivatives thereof to the corresponding 2-(N-p-methoxybenzyl)carboxamide according to known methods. The N-substituted amide is reacted with phosgene in pyridine for from about 2 to 5 hours to give an iminochloride. The iminochloride is treated with sodium azide in dry N,N-dimethylformamide at ambient temperature for from 2 to 4 hours to provide the 1,5-disubstituted tetrazole. The p-methoxybenzyl group is then removed by treating the substituted tetrazole with trifluoroacetic acid at about 40° C. and an equivalent of anisole. (The 4-oxo group, if converted to a 4-chloro group by phosgene, is regenerated by alkaline hydrolysis according to standard procedures.)

In still a further method, the 2-(5-tetrazolyl) derivatives are prepared by heating 5-carbethoxytetrazole (Oliveri-Mandola, Gazz. Chim. Ital. 41, I, 59 (1911)) with the appropriate 2-aminoquinolin-3-carboxamide, the 3-aminonaphthalene-2-carboxamide or the 2-aminonicotinamide reactant in an inert solvent. This procedure corresponds to the common step of Routes A and B above but wherein the Y-Z reactant is 5-carbethoxytetrazole.

The amino acid derived amides are conveniently obtained from the corresponding lower alkyl ester, e.g., ethyl ester, by reaction with the appropriate amino acid or derivative thereof in the manner described in Examples VII and IX below. The compound of formula I, N-carbethoxymethyl 7,8-dimethoxypyrimido-[4,5-b]quinolin-4(3H)-one-2-carboxamide (R₁, R₂, R₅ = H; R₃, R₄ = OCH₃ ; R^(o) = NHCH₂ COOC₂ H₅), when administered intravenously at 30 and 3 mg./kg. of body weight to rats affords 96 and 32% protection, respectively in the PCA test.

EXAMPLE I Ethyl Pyrimido[4,5-b]Quinolin-4(3H)-One-2-Carboxylate A. 2-Aminoquinoline-3-Carboxamide

Cyanoacetamide (19.4 g., 0.23 mole) is added to a solution of sodium ethoxide (6.65 g., 0.29 mole) in absolute ethanol (460 ml.) maintained at 50° C. The mixture is stirred and o-aminobenzaldehyde (28.0 g., 0.23 mole) in absolute ethanol (100 ml.) added. After fifteen minutes stirring at 50° C., the reaction mixture is cooled in an ice bath, filtered and dried to give 34.5 g. (84% yield) of product; m.p. 240°-242° C.

B. Ethyl Pyrimido[4,5-b]quinoline-4(3H)-one-2-carboxylate

A mixture of 2-aminoquinoline-3-carboxamide (25.0 g., 0.134 mole) and diethyl oxalate (500 ml.) is heated to reflux for 4 hours while distilling off the ethanol-water formed. The reaction mixture is then cooled to room temperature, the solid product filtered off, washed with diethyl oxalate and air dried to give 22.6 g. of brownish-green solid; m.p. 245°-246° C. It is purified by recrystallization, with decolorization, from hot chloroform. The off-white solid thus obtained (16.2 g., 45% yield) melts at 247°-248° C.

Analysis: Calcd. for C₁₄ H₁₁ N₃ O₃ : C, 62.51; H, 4.24; N, 15.67%.

Found: C, 62.44; H, 4.24; N, 15.67%.

The same compound is obtained by: (a) refluxing equimolar amounts of 1-carbethoxyformimidate and ethyl 2-aminoquinoline-3-carboxylate in ethanol for three hours. The reaction mixture is concentrated, the product collected by filtration and recrystallized from hot chloroform; or (b) refluxing equimolar amounts of 1-carbethoxyformamidine and 2-aminoquinoline-3-carboxamide in ethanol for 3-4 hours in the presence of an equimolar amount of sodium ethoxide. The reaction mixture is worked up as described in (a) above.

EXAMPLE II Ethyl 7,8-Dimethoxy pyrimido[4,5-b]Quinolin-4(3H)-One-2-Carboxylate A. α-Cyano-β-(2-Nitro-4,5-Dimethoxyphenyl)Acrylamide

Piperidine (2.1 g., 0.0237 mole) and 2-cyanoacetamide (22.0 g., 0.263 mole) are added to a slurry of 6-nitroveratraldehyde (50.0 g., 0.237 mole) in methanol (500 ml.). The mixture is heated to reflux for 2 hours, and then cooled in an ice bath and filtered. The bright yellow filter cake is washed with cold isopropanol (300 ml.) and then air dried. Yield = 60.1 g. (93%); m.p. 265°-266° C.

Analysis: Calcd. for C₁₂ H₁₁ N₅ O₃ : C, 51.99; H, 4.00; N, 15.16%

: c, 51.96; h, 4.20; n, 15.23%;

following the procedure of preparations above, but using the appropriate alkoxy substituted nitrobenzaldehyde reactant, the compounds listed below are prepared: ##STR4##R₂ R₃ R₄ R₅ m.p.(°C) % Yield__________________________________________________________________________H OCH₃ H OCH₃ 231-2 (dec.) 20.sup.(a)OCH₃ H H OCH₃ 247-8 (dec.) 82.5H H OCH₃ H 157-2 (dec.) 42H OCH₃ OCH₃ OCH₃ 244.5 (dec.) 73.5H OC₂ H₅ O--n--C₆ H₉ H 54H OC₂ H₅ OC₇ H₇ H 167-167.5 (dec.) 73H OC₂ H₅ OCH₃ H 243-4 (dec.) 62H OC₂ H₅ OC₂ H₅ H 191-3 (dec.) 79H --OCH₂ --CH₂ O-- H 301.5 (dec.) 98.2H OC₇ H₇ OCH₃ H 157-8 45H OCH₃ OC₇ H₇ H 182-3 52H OC₇ H₇ H H 125-6 47__________________________________________________________________________ .sup.(a) refluxed for 18 hours.

B. 6,7-Dimethoxy-2-Aminoquinoline-3-Carboxamide

Iron powder (65.2 g., 1.22 moles) is added over a period of one-half hour to a slurry of α-cyano-β-(2-nitro-4,5-dimethoxyphenyl)acrylamide (75.0g., 0.271 mole) in a 50% solution of acetic acid-N,N-dimethylformamide (750 ml.) at 75°C. When addition of the iron powder is complete, the mixture is heated to 90°C. for 4 hours and then filtered while hot. The filter cake is washed with hot acetic acid (150 ml.). The dark red filtrate is gradually added to IN hydrochloric acid (1500 ml.) and the pink precipitate recovered by filtration and recrystallized from an excess (10%) of aqueous sodium hydroxide. The solid is filtered, washed with cold isopropanol and dried to give the title product as yellow crystals. Yield = 57.1 g. (83.5%); mpp. 274°-275°C.

Analysis: Calcd. for C₁₂ H₁₅ N₃ O₃ : C, 58.30; H, 5.29; N, 16.99%.

Found: C, 58.06; H, 5.29; N, 17.25%

Upon repetition of procedure B above but using the appropriate α-cyano-β-(2-nitro-alkoxyphenyl)acrylamide from procedure A, the following compounds are prepared: ##STR5##R₂ R₃ R₄ R₅ m.p. (°C.) % Yield______________________________________H OCH₃ H OCH₃ 284-286 52OCH₃ H H OCH₃ 263-4(dec) 78.5H H OCH₃ H 281-2(dec) 82H OCH₃ OCH₃ OCH₃ 240-1.5(dec) 83.2H OC₂ H₅ O--n-C₄ H₉ H 252 85H OC₂ H₅ OC₇ H₇ H 279-80(dec) 73H OC₂ H₅ OCH₃ H 234-5(dec) 83.7H OC₂ H₃ OC₂ H₅ H 243-4(dec) 90.8H --OCH₂ CH₂ O-- H 269.5(dec) 26.5H OC₇ H₇ OCH₃ H 264-6 80H OCH₃ OC₇ H₇ H 282-3(dec) 95H OC₇ H₇ H H 238-9 94______________________________________

C. Ethyl 7,8-dimethoxypyrimido[4,5-b]quinolin-4(3H)-one-2-carboxylate

To a round-bottomed flask equipped with stirrer, reflux condenser and Dean-Stark apparatus and containing a mixture of diethyl oxalate (50 ml.) and xylene (80 ml.) at reflux is added 6,7-dimethoxy-2-aminoquinoline-3-carboxamide (3.0 g., 0.012 mole). The xylene, water and ethanol are distilled off and collected over a four hour period. When all the xylene is removed, the reaction mixture is brought to 185° C., cooled to about 100° C. and then slowly poured into chloroform (300 ml.). The chloroform solution is cooled and the brown precipitate which forms removed by filtration. The filtrate is decolorized with charcoal, concentrated and chilled to give a crystalline mass. The crystals are taken up in hot chloroform, the solution charcoalled, filtered and concentrated to give pale yellow crystals; 0.59 g. (15%); m.p. 273°--273° C. (dec.)

Analysis: Calcd. for C₁₆ H₁₅ N₃ O₅ : C, 58.41; H, 4.60; N, 12.77%.

Found: C, 57.91; H, 4.53; N, 12.37%.

An alternative route to this product comprises heating a mixture of ethyl 6,7-dimethoxy-2-aminoquinoline-3-carboxylate (1.38 g., 5.0 mM), ethyl 1-carbethoxyformimidate hydrochloride (1.82 g., 10 mM), triethylamine (5.1 g., 5.0 mM), ethanol (50 ml.) and N,N-dimethylformamide (20 ml.) on a steam bath for 17 hours. The reaction mixture is cooled and the solid which separates filtered off, triturated with hot chloroform and then recrystallized from chloroform to give 0.170 g. of product. M.P. 273°-274° C. (dec.).

A further alternative route is as follows: To ethyl oxalyl chloride (80.0 ml.) is added 6,7-dimethoxy-2-aminoquinoline-3-carboxamide (2.0 g., 0.81 mM) and the mixture is heated at 95° C. for 2 days. The reaction mixture is then cooled and filtered. The solid product is triturated with and recrystallized from hot chloroform.

Still further, a mixture of 6,7-dimethoxy-2-aminoquinoline-3-carboxamide (0.5 g., 2 millimoles), ethyl cyanoformate (0.43 g., 44 millimiles) and benzene (30 ml.) is refluxed for 3 days. The mixture is then cooled, filtered and the solid extracted with chloroform. Concentration of the chloroform extract affords the product which is recrystallized from chloroform.

Following the first procedure of C above, but using the appropriate alkoxy substituted 2-aminoquinoline-3-carboxamide reactant from B above and the appropriate ester of oxalic acid, the compounds listed below are prepared: ##STR6##R^(o) R₂ R₃ R₄ R₅ m.p.(°C) % Yield__________________________________________________________________________O--n-C₄ H₉ H OCH₃ OCH₃ H 263-4(dec) 14.5OC₂ H₅ H OCH₃ H OCH₃ 256-7(dec) 35OC₂ H₅ OCH₃ H H OCH₃ 254-5(dec) 24OC₂ H₅ H H OCH₃ H 263-4(dec) 9OC₂ H₅ H OCH₃ OCH₃ OCH₃ 250(dec) 31.4OC₂ H₅ H OC₂ H₅ O--n-C₄ H₉ H 201-3(dec) 16OC₂ H₅ H OC₂ H₅ OC₇ H₇ H 241-2(dec) 41OC₂ H₅ H OC₂ H₅ OCH₃ H 264-5(dec) 16OC₂ H₅ H OC₂ H₅ OC₂ H₅ H 243-4(dec) 19.1OC₂ H₅ H --OCH₂ CH₂ O-- H 252.5(dec) 11.2OC₂ H₅ H OC₇ H₇ OCH₃ H 274-5 25OC₂ H₅ H OCH₃ OC₇ H₇ H 254-5(dec) 8O--n-C₄ H₉ H OC₇ H₇ OCH₃ H 261-2(dec) 13OC₂ H₅ H OC₇ H₇ H H 256-7(dec) 32__________________________________________________________________________

D. 7,8-Dimethoxypyrimido[4,5-b]Quinolin-4(3H)-One-2-Carboxylic Acid

Ethyl 7,8-dimethoxypyrimido[4,5-b]quinolin-4(3H)-one-2-carboxylate (250 mg., 7.4 mM) in 5% aqueous sodium hydroxide (37.5 ml.) is stirred at room temperature for 20 hours. The ester dissolves within ten minutes and is followed by the gradual appearance of a light colored precipitate. The reaction mixture is acidified by the slow addition of 10% aqueous hydrochloric acid (13 ml.). The light colored precipitate dissolves and a yellow precipitate forms. The acid mixture is stirred for 45 minutes and then filtered. The filter cake is washed with water and then dried in vacuo. Yield = 140 mg. (63%) of yellow solid. M.P. 277°-280°C. (dec.). Analysis shows it to be 86% pure. The remainder is sodium chloride plus a small amount of water. It is purified by recrystallization from trifluoroacetic acid. The trifluoroacetate salt monohydrate thus obtained melts at 281°-283°C. (dec.).

Analysis: Calcd. for C₁₄ H₁₁ O₅ N₃. CF₃ COOH-H₂ O:

Calcd. C; 44.34; H, 3.25; N, 9.69; F, 13.15%

Found: C, 43.95; H, 3.17; N, 9.99; F, 13.75%

Ethyl 7-methoxy-8-ethoxypyrimido[4,5-b]quinoline-4(3H)-one-2-carboxylate is similarly prepared according to procedures A-C, beginning with 3-methoxy-4-ethoxy-6-nitrobenzaldehyde. It melts at 264°-265°C. (dec.)

It and the remaining products of Example II-C are hydrolyzed according to procedure D to afford the corresponding acids.

EXAMPLE III Ethyl 7-Fluoropyrimido[4,5-b]-Quinolin-4(3H)-One-2-Carboxylate A. α-Cyano-β-(2-Nitro-5-Fluorophenyl)Acrylamide

A mixture of 3-fluoro-6-nitrobenzaldehyde (10.0 g., 0.0592 mole), 2-cyanoacetamide (5.24 g., 0.0622 mole), piperidine (0.37 g., 4.36 millimole) and ethanol (92 ml.) is heated to reflux on a steam bath for 2 hours. It is then cooled in an ice-bath whereupon the product precipitates and is recovered by filtration, washed with cold ethanol and dried. Yield = 9.1 g. (65%) of crude product. M.P. 162°-166° C.

It is of sufficient purity for use in the succeeding step.

B. 6-Fluoro-2-Aminoquinoline-3-Carboxamide

Iron powder (8.52 g., 0.152 mole) is gradually added over a 40-minute period to a slurry of α-cyano-β-(2-nitro-5-fluorophenyl)acrylamide (7.97 g., 0.034 mole) in acetic acid (100 ml.) at 85° C. When addition of the iron powder is complete, the mixture is heated to 95°-100° C. for 1.5 hours and then filtered hot through diatomaceous earth. The filtrate is cooled in an ice bath and then filtered to give a tan crystalline solid. The solid is partitioned between ethyl acetate and water, the organic phase separated, dried over anhydrous sodium sulfate and concentrated to yield 6.34 g. (90%) of the title amide as yellow crystals. M.P. 232°-236° C.

C. Ethyl 7-fluoropyrimido[4,5-b]quinolin-4(3H)-one-2-carboxylate

A mixture of diethyl oxalate (57.2 g., 0.392 mole) and 6-fluoro-2-aminoquinoline-3-carboxamide (5.34 g., 0.0261 mole) is heated for 18 hours at 160° C. under a nitrogen atmosphere and then allowed to cool to room temperature. Hexane (300 ml.) is added, the mixture stirred and filtered to provide the desired product. It is washed with hexane and dried. Yield = 4.9 g. (71%); m.p. 272° C. (dec.).

Analysis: Calcd. for C₁₉ H₁₀ FN₃ O₃ : C, 58.54; H, 3.51; N, 14.63; F, 6.61%.

Found: C, 58.29; H, 3.47; N, 14.69; F, 6.70%.

EXAMPLE IV Ethyl 8-Fluoropyrimido[4,5-b]Quinolin-4(3H)-One-2-Carboxylate A. α-Cyano-β-(2-Nitro-4-Fluorophenyl)Acrylamide

A mixture of 4-fluoro-2-nitrobenzaldehyde (8.70 g., 51.5 millimoles), 2-cyanoacetamide (4.7 g., 54.4 millimoles), piperidine (0.032 g., 0.37 millimole) and ethanol (80 ml.) is stirred at room temperature for 28 hours. The solid which formed is separated by filtration, washed with ethanol (45 ml.) and dried. Yield = 10 g. (83.5%) of the title acrylamide as light tan crystals. M.P. 278°-279° C.

B. 7-Fluoro-2-Aminoquinoline-3-Carboxamide

To a solution of the product of Preparation A above (10.0 g., 0.042 mole) in acetic acid (192 ml.) at 110° C. is gradually added over a 40 minute period, iron powder (10.3 g., 0.192 mole). The mixture is stirred for one hour and then concentrated under reduced pressure to a thick paste. The paste is partitioned between ethyl acetate (100 ml.) and water (100 ml.) and the phases separated. This partition step is repeated three more times. The combined ethyl acetate layers are dried (Na₂ SO₄), filtered and concentrated to small volume. The solid is filtered off and dried. Yield = 4.72 g. (57.3%) of crude product; m.p. 259°-262° C. It is used directly in the succeeding step.

C. Ethyl 8-fluoropyrimido[4,5-b]quinolin-4(3H)-one-2-carboxylate

Diethyl oxalate (47.5 g., 0.326 mole) and 7-fluoro-2-aminoquinoline-3-carboxamide (3.7 g., 0.015 mole) are mixed together and heated at 160° C. for seven hours. The reaction mixture is cooled to room temperature, hexane (300 ml.) added, and the brown solid which precipitates filtered off, and washed with hexane (60 ml.). It is then slurried in hot chloroform (250 ml.) and the insoluble material removed by filtration. The hot filtrate is decolorized with charcoal, filtered and dried (Na₂ SO₄). The light brown solid which precipitates upon cooling is filtered off and recrystallized from isopropyl alcohol. Yield = 1.04 g. (29.2%) of the title ethyl ester. M.P. 248°-251° C.

Analysis: Calcd. for C₁₄ H₁₀ FN₃ O₃ : C, 58.54; H, 3.51; N, 14.63; F, 6.61%.

Found: C, 58.52; H, 3.61; N, 14.76; F, 6.56%.

EXAMPLE V Ethyl 7-Chloropyrimido[4,5-b]Quinolin-4(3H)-One-2-Carboxylate A. α-Cyano-β-(2-Nitro-5-Chlorophenyl)Acrylamide

A mixture of 5-chloro-2-nitrobenzaldehyde (47.0 g., 0.25 mole), 2-cyanoacetamide (21.0 g., 0.25 mole), diethylamine (0.5 ml.) and ethanol (500 ml.) is heated at reflux for one hour. Sodium ethoxide (10 mg.) is added to the clear mixture and refluxing continued for an additional 2.5 hours. It is then cooled and the crystalline product which precipitates recovered by filtration. Yield = 52.3 g. (88%); m.p. of crude = 183°-185° C. It is used without purification in step B.

B. 6-Chloro-2-Aminoquinoline-3-Carboxamide

Following the procedure of Example IV B, α-cyano-β-(2-nitro-5-chlorophenyl)acrylamide (51.0 g., 0.2 mole) is treated with iron powder (56 g., 1.0 mole) to provide 19 g. (43%) of the title carboxamide derivative. M.P. 250°-251° C. when recrystallized from ethanol.

C. Ethyl 7-Chloropyrimido[4,5-b]quinolin-4(3H)-one-2-carboxylate

A mixture of 6-chloro-2-aminoquinoline-3-carboxamide (2.2 g., 0.01 mole), sodium hydride (0.52 g., 0.012 mole as 56% dispersion in oil) and N,N-dimethylformamide (20 ml.) is added to a solution of diethyl oxalate (5 ml.) and N,N-dimethylformamide (5 ml.). The reaction mixture is stirred for fifteen minutes, a second portion of sodium hydride (0.52 g., 0.012 mole) added and stirring continued for an additional fifteen minutes. Acetic acid (25.0 ml.) is cautiously added to the mixture followed by water (50 ml.). The tan precipitate is removed by filtration and dried (2.0 g. of crude ester). It is purified by chromatography on silica gel using chloroform:2B ethanol (99:1) as eluant. The product, recovered by concentration of the eluate, is recrystallized from acetic acid. Yield = 1.3 g. (37.3%). M.P. 260.5° C. (dec.). Mass spectra gave m/e = 303.

EXAMPLE VI Ethyl 7,8-Methylenedioxypyrimido[4,5-b]Quinolin-4(3H)-One-2-Carboxylate A. 6,7-Methylenedioxy-2-Aminoquinoline-3-Carboxamide

To sodium methoxide (1.90 g., 0.03 mole) in methanol (50 ml.) is added 4,5-methylenedioxy-2-aminobenzaldehyde (5.0 g., 0.03 mole) and 2-cyanoacetamide (2.50 g., 0.03 mole). The mixture is heated to reflux for 15 minutes and then cooled in an ice bath. The bright yellow solid is filtered off and recrystallized from acetic acid to give 6.18 g. (89.3%) of the desired product. M.P. 308° C. (dec.). (m/e = 231)

B. Cyclization of 6,7-methylenedioxy-2-aminoquinoline-3-carboxamide

A mixture of 6,7-methylenedioxy-2-aminoquinoline-3-carboxamide (1.50 g., 6.5 millimoles), sodium methoxide (0.05 g., 1.25 millimoles) and diethyl oxalate (150 ml.) is heated to 150° C. for 3.5 hours. The mixture is then cooled to room temperature to precipitate the product which is recovered by filtration (1.0 g.). It is purified by chromatography on silica gel using chloroform:methanol (99:1) as eluant. The product is recovered by evaporation of the eluate and recrystallization of the residue from ethanol. Yield = 0.414 g. (20.4%) of the cyclized product. M.P. 267° C. (dec.).

Analysis: Calcd. for C₁₅ H₁₁ N₃ O₅ : C, 57.51; H, 3.54; N, 13.41%.

Found: C, 57.19; H, 3.67; N, 13.33%.

EXAMPLE VII 7,8-Dimethoxypyrimido[4,5-b]Quinolin-4(3H)-One-2-Carboxamide

Anhydrous ammonia is bubbled into a mixture of ethyl 7,8-dimethoxypyrimido[4,5-b]quinolin-4(3H)-one-2-carboxylate (300 mg., 9.00 millimoles) in absolute ethanol (75 ml.) for fifteen minutes. A clear solution formed followed after a few minutes by formation of a precipitate. The reaction mixture is transferred to a pressure bomb (Monel) and heated in a 95° C. oil bath overnight. The bomb is then cooled to room temperature and the contents removed. The bomb is washed with ethanol and the combined reaction mixture plus wash filtered to recover the product. The filter cake is washed with ethanol and then dried in air. Yield = 260 mg. (95%). M.P. 310° C. (dec.).

EXAMPLE VIII n-Butylpyrimido[4,5-b]Quinolin-4(3H)-One-2-Carboxylate

Ethylpyrimido[4,5-b]quinolin-4(3H)-one-2-carboxylate (3.0 g., 1.12 millimoles) is added to n-butanol (350 ml.) containing ten drops of hydrochloric acid. The mixture is refluxed for 36 hours, and then cooled and filtered. The solid is dissolved in hot chloroform, the insoluble matter removed by filtration and the filtrate concentrated to provide 1.19 g. of the n-butyl ester (36% yield). M.P. 218°-219.5° C.

Analysis: Calcd. for C₁₆ H₁₅ N₃ O₃ : C, 64.71; H, 5.09%.

Found: C, 64.12; H, 5.20%.

EXAMPLE IX Pyrimido[4,5-b]Quinolin-4(3H)-One-2-Carboxamide

A mixture of 2-aminoquinoline-3-carboxamide (2.0 g., 1.15 millimoles), ethyl oxamate (2.71 g., 23.1 millimoles), ethylene glycol (10 ml.) and sodium methoxide (10 mg.) is heated at 170° C. for 1 hour. The product is precipitated by slow addition of ice cold methanol (50 ml.) to the hot reaction mixture followed by chilling in an ice bath. It is filtered off, washed with cold methanol and dried in vacuo. Yield = 0.768 g. (28%). M.P. 320° C.

Analysis: Calcd. for C₁₂ H₈ O₂ N₄ : C, 59.96; H, 3.35; N, 23.32%.

Found: C, 59.90; H, 3.43; N, 22.10%.

EXAMPLE X Ethyl 6-Chloropyrimido[4,5-b]Quinolin-4(3H)-One-2-Carboxylate A. 5-Chloro-2-Aminoquinoline-3-Carboxamide

Following the procedure of Example I-A, 2-amino-6-chlorobenzaldehyde (5.4 g., 0.035 mole), 2-cyanoacetamide (2.94 g., 0.035 mole), sodium ethoxide (3.0 g., 0.044 mole) in ethanol (50 ml.), the title carboxamide is prepared in 77% yield (5.92 g.). M.P. 270°-273° C. (dec.).

B. Ethyl 6-Chloropyrimido[4,5-b]quinolin-4(3H)-One-2-Carboxylate

Diethyl oxalate (350 ml.) and 5-chloro-2-aminoquinoline-3-carboxamide (5.9 g., 0.0267 mole) are heated together at 170° C. for 4 hours. By-product water and ethanol are distilled from the reaction mixture. The dark reaction mixture is cooled, treated with hexane (300 ml.) and filtered. The brown solid is dissolved in methylene chloride:ethanol (1:1), decolorized with charcoal and concentrated to give a tan solid. The solid is recrystallized from methylene chloride-hexane. It is purified further by chromatography on silica gel using methylene chloride:ethanol (9:1) as eluant. The product, recovered by evaporation of the eluate, is recrystallized from methylene chloride-ethanol. Yield = 0.756 g. (9.3%). M.P. 278°-279° C. (dec.).

Analysis: Calcd. for C₁₄ H₁₀ ClN₃ O₃ : C, 55.50; H, 3.33; N, 13.87%.

Found: C, 55.03; H, 3.21; N, 13.85%.

EXAMPLE XI Pyrimido[4,5-b]Quinolin-4(3H)-One-2-Carboxylic Acid Disodium Salt

Ethyl pyrimido[4,5-b]quinolin-4(3H)-one-2-carboxylate (5.0 g., 0.0185 mole) is added to 15% aqueous sodium hydroxide (200 ml.) and the mixture stirred for twenty hours at room temperature. The yellow solid which formed is filtered from the reaction mixture, dissolved in water, and the solution adjusted to pH 7 by slow addition of 10% hydrochloric acid. The precipitate is filtered and triturated with methanol. The slurry is filtered, the filter cake washed with methanol and dried. Yield = 4.0 g. (73.4%) of the disodium hemi-hydrate salt. M.P. 345°-347° C. (dec.).

EXAMPLE XII Ethyl 7-Methoxypyrimido[4,5-b]Quinolin-4(3H)-One-2-Carboxylate A. α-Cyano-β-(5-Methoxy-2-Nitrophenyl)Acrylamide

Following the procedure of Example II-A, 5-methoxy-2-nitrobenzaldehyde is converted to the title acrylamide derivative in 86% yield. M.P. 207°-208° C. (dec.)

B. 6-Methoxy-2-Aminoquinoline-3-Carboxamide

α-Cyano-β-(5-methoxy-2-nitrophenyl)acrylamide is reacted according to the procedure of Example II-B to give the corresponding quinoline derivative in 79.5% yield. M.P. 231°-232° C. (dec.).

C. Ethyl 7-Methoxypyrimido[4,5-b]-Quinolin-4(3H)-One-2-Carboxylate

6-Methoxy-2-aminoquinoline-3-carboxamide is converted to the title compound by the procedure of Example II-C in 10% yield. M.P. 263°-264° C. (dec.).

Analysis: Calcd. for C₁₅ H₁₃ N₃ O₄ : C, 60.19; H, 4.38; N, 14.04%.

Found: C, 60.28; H, 4.34; N, 14.26%.

EXAMPLE XIII Ethyl 8,9-Dimethoxypyrimido[4,5-b]-Quinolin-4(3H)-One-2-Carboxylate

The procedures of Example II-A-C are repeated but using 2-nitroveratraldehyde (2.08 g.) in place of 6-nitroveratraldehyde. The yields of melting points of the intermediates and final product are listed below:

A. α-cyano-β-(2-nitro-3,4-dimethoxyphenyl)acrylamide: Yield = 64%; M.P. 194°-195° C.

B. 7,8-dimethoxy-2-aminoquinoline-3-carboxamide: Yield = 50%; M.P. 218°-219° C.

C. ethyl 8,9-dimethoxypyrimido[4,5-b]-quinolin-4(3H)-one-2-carboxylate; Yield = 30%; M.P. 233°-234° C. (dec.).

Analysis: Calcd. for C₁₆ H₁₅ N₃ O₃ : C, 58.35; H, 4.60; N, 12.76%.

Found: C, 58.52; H, 4.47; N, 12.68%.

EXAMPLE XIV 7,8-Dimethoxypyrimido-[4,5-b]-Quinolin-4(3H)-One-2-Hydroxamic Acid

Ethyl 7,8-dimethoxypyrimido-[4,5-b]quinolin-4(3H)-one-2-carboxylate (200 mg., 6.08 millimoles) is added to a solution of hydroxylamine hydrochloride (69 mg., 100 millimoles) and triethylamine (100 mg., 100 millimiles) in absolute ethanol (50 ml.). The mixture is heated at 95° C. overnight in a bomb and then cooled. The insoluble yellow solid is filtered off, washed with hot ethanol and dried to provide 143 mg. (74%) of the title product. M.P. 337° C. (dec.).

EXAMPLE XV Ethyl 7-Hydroxy-8-Methoxypyrimido[4,5-b]Quinolin-4(3H)One-2-Carboxylate Trifluoroacetate

A solution of ethyl 7-benzyloxy-8-methoxypyrimido[4,5-b]quinolin-4(3H) one-2-carboxylate (250 mg., 0.618 mmole) in trifluoroacetic acid (5 ml.) is refluxed for 2.5 hours. (Alternatively the mixture is stirred at room temperature for three days). The reaction mixture is then poured into ether (25 ml.) and the resulting bright yellow precipitate of the trifluoroacetate salt recovered by filtration, washed with ether and dried. Yield = 194 mg., 72%; m.p. 279°C.

Analysis:

Calcd. for C₁₅ H₁₃ N₃ O₅ CF₃ COOH 1/2 H₂ O: C, 46.58; H, 3.42; N, 9.58%

Found: C, 46.81; H, 3.41; N, 9.21%

Repetition of the above procedure but using the corresponding n-butyl ester (862 mg., 0.199 mmole) in place of the ethyl ester, 8 ml. trifluoroacetic acid and a reflux period of 3.5 hours affords n-butyl 7-hydroxy-8-methoxypyrimido[4,5-b]quinolin-4(3H)one-2-carboxylate trifluoroacetate (0.67 g., 74%); m.p. 240°-241°C. (dec.)

Analysis: Calcd. for C₁₇ H₁₇ O₅ N₃ CF₃ COOH: C, 49.89; H, 3.96; N, 9.18; F. 12.46% C, 49.93; H, 3.97; N, 8.79; F. 11.17%.

Similarly, ethyl 7-benzyloxypyrimido-[4,5-b]quinolin-4(3H) one-2-carboxylate is debenzylated to give a 74% yield of ethyl 7-hydroxypyrimido [4,5-b]quinolin-4(3H)one-2-carboxylate hemitrifluoroacetate; m.p. 274°-275°C. (dec.)

Analysis: Calcd. for C₁₄ H₁₁ O₄ N₃ 1/2 CF₃ COOH; C, 57.43; H, 3.66; N. 13.39% C, 57.75; H, 4.05; N, 13.65%.

EXAMPLE XVI 7-Hydroxy-8-Methoxypyrimido[4,5-b]Quinolin-4(3H)One-2-Carboxylic Acid

The trifluoroacetate-hemihydrate salt of ethyl 7-hydroxy-8-methoxypyrimido[4,5-b]quinolin-4(3H)one-2-carboxylate (200 mg., 0.456 mmole) is slurried in 0.1N sodium hydroxide (5 ml.) and 15% sodium hydroxide added dropwise with stirring until solution is complete. The mixture is stirred at room temperature for 18 hours and is then made strongly acid by addition of trifluoroacetic acid. The precipitate which forms is separated by filtration, washed with water and then with isopropyl alcohol and air-dried. Yield = 114 mg.; m.p. 281°C. (dec).

Analysis: Calcd. for C₁₃ H₉ O₅ N₃ 2H₂ O: C, 48.29; H, 4.02; N, 13.00%

Found: C, 47.3; H, 3.32; N, 12.73%

In like manner, ethyl 7-hydroxypyrimido[4,5-b]quinolin-4(3H)one-2-carboxylate hemitrifluoroacetate (210 mg.) is converted to 7hydroxypyrimido[4,5-b]quinolin-4(3H)one-2-carboxylic acid. Yield - 191 mg., 91%; m.p. 340°C (dec.)

EXAMPLE XVII Ethyl 7-Methoxy-8-Hydroxypyrimido[4,5-b]Quinolin-4(3H)One-2-Carboxylate

A solution of ethyl 7-methoxy-8-benzyloxypyrimido[4,5-b]quinolin-4(3H)one-2-carboxylate (198 mg., 0.488 mmole) in trifluoroacetic acid (4 ml.) is stirred at room temperature for 8 days. Ether (15 ml.) is added and the yellow precipitate which forms separated by filtration. It is taken up in chloroform and the solution filtered through diatomaceous earth to remove a small amount of insoluble material. The filtrate is decolorized with charcoal and concentrated under reduced pressure to small volume. The solid which forms is filtered and dried; 9 mg., 6% yield; m.p. 265°-266°C. (dec.).

EXAMPLE XVIII

The compounds below are prepared from appropriate reactants by the procedures of the preceding examples. ##STR7## MethodR^(o) R₂ R₃ R₄ R₅ of Ex.______________________________________OCH₃ H H H H INHOH H H H H XIVOCH₃ H F H H IIINH₂ H F H H VIIO--n-C₄ H₉ H H F H VIIINH₂ H H F H VIINH₂ Cl H H H VIINHOH Cl H H H XIVO--i-C₃ H₇ H Cl H H VNH₂ H Cl H H IXOCH₃ H --O--CH₂ --O-- H VINH₂ H --O--CH₂ --O-- H IXNHOH H --O--CH₂ --O-- H XIV OCH₃ H OCH₃ OCH₃ H IIO--n-C₃ H₇ H OCH₃ OCH₃ H IINH₂ H OCH₃ OCH₃ H IXNH₂ H OCH₃ H H VIINH₂ H H OCH₃ OCH₃ VIIOC₂ H.sub. 5 H OC₂ H₅ H H IIOC₂ H₅ H H OC₂ H₅ H IINH₂ H OC₂ H₅ OC₂ H₅ H VIINHOH H OC₂ H₅ OC₂ H₅ H XIVOC₂ H₅ H Br H Br IOC₂ H₅ H Cl H Cl IOCH₃ Cl H H Cl IOC₂ H₅ H H H OCH₃ IINH₂ H H H OCH₃ VIINH₂ H H OC₂ H₅ H VIIOCH₃ H H Cl H VO-- n-C₃ H₇ OCH₃ H H OCH₃ IIOC₂ H₅ H H I H IIOC₂ H₅ Br H H H VNH₂ Br H H H VIIOC.sub. 2 H₅ OCH₃ --O--CH₂ --O-- H INH₂ OCH₃ --O--CH₂ --O-- H VIIO--i-C₃ H₇ H CH₃ H H VIIINH₂ H CH₃ H H VIIOC₂ H₅ H H CH₃ H IOC₂ H₅ CH₃ H H H INH₂ CH₃ H H H IXNHOH CH₃ H H H XIVOC₂ H₅ H t-C₄ H₉ H H INH₂ H t-C₄ H₉ H H VIIOCH₃ CH₃ H CH₃ H IOC₂ H₅ CH₃ CH₃ H H INH₂ CH₃ CH₃ H H VIIOC₂ H₅ H C₂ H₅ H H INH₂ H C₂ H₅ H H IXNHOH H n-C₃ H₇ H H XIVOC₂ H₅ H n-C₃ H₇ n-C₃ H₇ H VIINH₂ H n-C₃ H₇ n-C₃ H₇ H IXOC₂ H₅ H H C₂ H₅ H IOC₂ H₅ H H i-C₃ H₇ H IOC₂ H₅ --O--CH₂ --O-- OCH₃ H IO--n-C₄ H₉ --O--CH₂ --O-- OCH₃ H VIIINH₂ --O--CH₂ --O-- OCH₃ H VIINH₂ --O--CH₂ --O-- H H VIINH₂ H --O--CH₂ --CH₂ --O-- H IIOCH₃ OCH₃ OCH₃ OCH₃ H INH₂ OCH₃ OCH₃ OCH₃ H VIIO--n-C₃ H₇ H CH₃ CH₃ H IOC₂ H₅ Br H H H IOC₂ H₅ H Br H H INH₂ H Br H H VIIOC₂ H₅ H H Br H INHOH H H Br H XIVOCH₃ H H t-C₄ H₉ H IIOC₂ H₅ H OCH₃ OC₂ H₅ H IIOCH₃ H Cl Br Cl IIOCH₃ H H O--i-C₃ H₇ OCH₃ IIOC₂ H₅ OCH₃ --O--CH₂ --O-- OCH₃ IIOC₂ H₅ H O--n-C₄ H₉ O--n-C₄ H₉ H IIOC₂ H₅ H H Cl H IIOC₂ H₅ H H Cl Cl IIO--n-C₄ H₉ H SCH₃ H H IINH₂ H SCH₃ H H VIIOCH₃ H SCH₃ SCH₃ H IINHOH H SCH₃ SCH₃ H XIVOC₂ H₅ H H H SCH₃ IIOC₂ H₅ H H OC₇ H₇ OCH₃ IIO--n-C₃ H₇ H H OCOC₇ H₇ H IIO--n-C₄ H₉ F H H H IIOCH₃ OC₇ H₇ OCH₃ H H IIOC₂ H₅ H OC₇ H₇ OC₇ H₇ H IIOC₂ H₅ OC₇ H₇ H H H IIOC.sub. 2 H₅ OC₇ H₇ Br H Br IIOCH₃ H OCH₃ OC₇ H₇ Br IIOCH₃ H OC₇ H₇ OC₇ H₇ H IIOC₂ H₅ OC₇ H₇ OCH₃ OCH₃ H IIOC₂ H₅ H Br H OC₇ H₇ IO--n-C₄ H₉ H H H OC₇ H₇ IO--n-C₃ H₇ C₂ H₅ H OC₇ H₇ H IIIO--n-C₃ H₇ C₂ H₅ OC₇ H₇ H H IIIOC₂ H₅ H OC₇ H₇ OC₂ H₅ H IIOC₂ H₅ H OC₂ H₅ OC₇ H₇ H IIOC₂ H₅ H F OC₇ H₇ H IIOC₂ H₅ H Br OC₇ H₇ H IIOCH₃ H Cl OC₇ H₇ H VIINH₂ H OC₇ H₇ H OCH₃ VIINH₂ H OCH₃ OC₇ H₇ H IXNHOH H OCH₃ OC₇ H₇ H XIVNHOH H OC₇ H₇ OCH₃ H XIVNHOH OC₇ H₇ OCH₃ OCH₃ H XIVNHOH H OC₂ H₅ OC₇ H₇ H XIVNH₂ H OC₇ H₇ OC₂ H₅ H VIINH₂ H F OC₇ H₇ H VIINHOH H Cl OC₇ H₇ H XIVNH₂ H OC₇ H₇ OCH₃ H VIINH₂ H Cl OCH₃ H VIIOC₂ H₅ H SC₇ H₇ H H IINH₂ H H H SC₇ H₇ VIIOC₂ H₅ H SC₇ H₇ C₂ H₅ H IIOC₂ H₅ H H SC₇ H₇ H IINHOH H H SC₇ H₇ H XIVOC₂ H₅ SC₇ H₇ H H SC₇ H₇ IINH₂ SC₇ H₇ H H SC₇ H₇ XI______________________________________

The benzyl ethers and benzylthio ethers tabulated above are converted to the corresponding hydroxy and thiol compounds by the procedure of Example XV.

The thus-produced hydroxy and thiol substituted 2-carboxylic acid esters are hydrolyzed to the corresponding 2-carboxylic acid derivatives by the procedure of Example XVI.

EXAMPLE XIX Ethyl 7-Acetoxy-8-Methoxypyrimido[4,5-b]Quinolin-4(3H)One-2-Carboxylate p-Toluenesulfonate

A mixture of acetic anhydride (4 ml.), ethyl 7-hydroxy-8-methoxy-pyrimido[4,5-b]quinolin-4(3H)one-2-carboxylate trifluoroacetate hemihydrate (250 mg. 0.572 mmole) and p-toluenesulfonic acid monohydrate (100 mg., 0.572 mmole) is heated at 100°C. for 24 hours. The acetic anhydride is then stripped from the reaction mixture in vacuo. The solid residue is dissolved in hot chloroform and the solution decolorized with activated charcoal. Benzene (4 volumes) is added to the decolorized solution which is then chilled in ice. The crystals which separate are recovered by filtration and air dried. Yield = 174 mg., 58%; m.p. 215°-217°C.

Analysis: Calcd. for C₁₇ H₁₅ O₆ N₃ C₇ H₈ O₃ S: C, 54.43; H, 4.37; N, 7.98% Found: C, 53.74; H, 4.28; N, 7.24%

The above procedure is repeated but using the corresponding n-butyl ester trifluoroacetate salt (200 mg., 0.437 mmole) in place of the ethyl ester trifluoroacetate salt. The product is recovered by concentration of the chloroform solution to 1/3 volume and filtration of the solid which precipitates rather than by precipitation with benzene. Yield - 30 mg., 18%; m.p. 260°-261°C. (dec.)

The above procedure is repeated but using the appropriate acid anhydride and in place of acetic anhydride and the appropriate hydroxy or thiol formula I compound as reactant to give the following compounds: ##STR8##R₁ R₂ R₃ R₄ R₅ R__________________________________________________________________________H H C₂ H₅ COO OCH₃ H COOC₂ H₅H H C₃ H₇ COO OCH₃ H COOC₂ H₅H H HCOO OCH₃ H COOC₂ H₅H H OCH₃ CH₃ COO H COOC₂ H₅H H OCH₃ C₂ H₅ COO H COOC₂ H₅H H OCH₃ HCOO H COOC₂ H₅H H HCOO OCH₃ H COO--n-C₄ H₉H H CH₃ COO OCH₃ H COO--n-C₄ H₉C₆ H₅ H HCOO H H CH₃C₆ H₅ H C₃ H₇ COO OCH₃ H CH₃H H H HCOO H COO--n-C₃ H₇H H H CH₃ COO H COO--n-C₃ H₇C₆ H₅ H H CH₃ COO OCH₃ CH₃H H CH₃ COO CH₃ COO H COOC₂ H₅H H HCOO HCOO H COOC₂ H₅H H OCH₃ CH₃ COO Br COOCH₃H CH₃ COO OCH₃ H H C₂ H₅CH₃ H C₆ H₅ COO OCH₃ H C₂ H₅n-C₄ H₉ H C₆ H₅ COO OCH₃ H C₂ H₅C₆ H₅ H CH₃ COO H H C₂ H₅H C₃ H₇ COO OCH₃ H H COOCH₃H CH₃ COO OCH₃ OCH₃ H COOC₂ H₅H H Br H CH₃ COO COOC₂ H₅H H H H HCOO COO--n-C₄ H₉H H H H C₃ H₇ COO COO--n-C₄ H₉H C₂ H₅ H CH₃ COO H COOC₂ H₅CH₃ H CH₃ COO OC₂ H₅ H CH₃C₂ H₅ H F CH₃ COO H C₂ H₅n-C₄ H₉ C₂ H₅ CH₃ COO H H C₂ H₅H H Cl CH₃ COO H COOCH₃C₂ H₅ H F C₃ H₇ COO H COOC₂ H₅H C₂ H₅ HCOO H H COO--n-C₃ H₇H H CH₃ COO H H COO--n-C₃ H₇H H C₃ H₇ COO H H COO--n-C₃ H₇H H CH₃ COO H H COOCH₃H H C₃ H₇ COO H H COOCH₃H H CH₃ COO CH₃ COO H COOCH₃H H H C₂ H₅ COO H COOC₂ H₅CH₃ H C₆ H₅ COO OCH₃ H COOC₂ H₅H H OCH₃ C₆ H₅ COO H COOC₂ H₅H H Cl C₆ H₅ COO H COOC₂ H₅__________________________________________________________________________

EXAMPLE XX 2-Hydroxyethyl 7,8-Dimethoxy-pyrimidin[4,5-b]quinolin-4-(3H)-One-2-Carboxylate

Triethylamine (1 ml.) is added to a slurry of ethyl 7,8-dimethoxypyrimidin [4,5-b]quinoline-4(3H)-One-2-carboxylate (500 mg.) in ethylene glycol (5 ml.). The mixture is stirred for 6 hours and is then diluted with water (30 ml.). The resulting clear yellow solution is acidified with acetic acid and the precipitate which forms filtered off. It is recrystallized while still damp from N,N-dimethylformamide (20 ml.). Yield = 285 mg. (53.5%). m.p. 252°C.

Analysis: Calcd. for C₁₆ H₁₅ N₃ O₆ : C, 55.65; H, 4.48; N, 12.17%. Found: C, 55.35; H, 4.51; N, 12.39%

In like manner, the alkyl esters of Example II-XIX, XXI-XXIII and XXVII-XXXII are transesterified to the corresponding 2-hydroxyethyl esters. Replacement of ethylene glycol by propylene glycol, butylene glycol or 1,4-dihydroxybutane affords the corresponding hydroxyalkyl esters.

EXAMPLE XXI Ethyl 7-Methylthiopyrimido[4,5-b]quinolin-4(3H)-One-2-Carboxylate A. α-Cyano-β-(2-Nitro-5-Methylthiophenyl)acrylamide

A solution of the sodium salt of methylmercaptan (2.78g., 0.0398 Mole) in N,N-dimethylformamide (50 ml.) is prepared by bubbling methylmercaptan into a mixture of sodium hydride (1.67 g., of 57% NaH) in N,N-dimethylformamide (50 ml.). The reaction mixture is cooled by means of an ice-bath until the reaction is complete.

The sodium methylmercaptide solution is then added dropwise to a mixture of α-cyano-β-(2-nitro-5-chlorophenyl)acrylamide (10 g., 0.0398 mole) in N,N-dimethylformamide (35 ml.) cooled in an ice-bath. The mixture is stirred for one hour and then removed from the ice-bath and stirred for an additional two hours. The reaction mixture is poured into water (600 ml.) and the resulting mixture thoroughly stirred. Ether (30 ml.) is added and the precipitate filtered off, washed with ether and dried. Yield = 8.4 g.; M.P. = 227°-229°C.

B. 6-Methylthio-2-Aminoquinoline-3-Carboxamide

A mixture of α-cyano-β-(2-nitro-5-methylthiophenyl)acrylamide (8.4 g., 0.032 mole) in acetic acid - N,N-dimethylformamide (110 ml. of 1:1) is heated in a 75°C. bath. Iron powder (2.0 g.) is added and the mixture stirred until the internal temperature rose to 95°C. Additional iron powder (6.16 g. total iron added=0.146 mole) is added in small portions over a fifteen-minute period. The reaction mixture is stirred for one hour following completion of addition and then filtered hot. The iron residue is washed with hot acetic acid and the combined filtrate and wash solution poured into IN hydrochloric acid (200 ml.) The hydrochloride salt which separates is filtered off and dissolved in hot dilute sodium hydroxide. The yellow solid which separates on cooling is filtered off, washed with isopropyl alcohol and air-dried. Yield = 3.26 g. (44%), M.P. 229°-242°C.

It is used in Step C without further purification.

C. Ethyl 7-methylthiopyrimido[4,5-b]quinoline-4(3H)-one-2-carboxylate

A round-bottom flask equipped with stirrer, condenser, thermometer and Dean-Stark trap and containing a mixture of 6-methylthio-2-aminoquinoline-3-carboxamide (1.0 g., 4.3 millimoles), diethyl oxalate (20 ml.) and xylene (15 ml.) is immersed in an oil-bath heated to 200°C. Xylene, water and ethanol are distilled from the reaction mixture until the internal temperature of the mixture reaches 165°C. The mixture is stirred at 165°C. for three hours and is then poured into chloroform (40 ml.) After cooling to room temperature the mixture is filtered. The brown filter cake is air-dried and then slurried in hot chloroform (100 ml.) to which activated charcoal is added. The slurry is filtered hot and the filtrate evaporated to half-volume and chilled in ice. The yellow solid which precipitates is filtered and dried in air; 142 mg. (11%). M.P. 240°-242°C.

Analysis: Calcd. for C₁₅ H₁₃ N₃ O₃ S: C, 57.2; H, 4.16; N, 13.3%

Found: C, 56.72; H, 4.13; N, 13.22%

EXAMPLE XXII Ethyl 7-Methylsulfinylpyrimido[4,5-b]quinoline-4(3H)-One-2-Carboxylate

A solution of ethyl 7-methylthiopyrimido[4,5-b]quinoline-4(3H)-one-2-carboxylate (315 mg., 1 millimole) in trifluoroacetic acid (2 ml.) is heated in an oil bath to 55°C. Hydrogen peroxide (113 mg. of 30% H₂ O₂, 1 millimole) is added and the solution stirred for ten minutes. After cooling to room temperature, absolute ethanol (6 ml.) is added. The resulting yellow precipitate is filtered off, washed with ether and air-dried. Recrystallization from absolute ethanol gives 185 mg. (56%) of product; m.p. 257°-259°C.

Analysis: Calcd. for C₁₅ H₁₃ N₃ O₄ S; C, 54.4; H, 3.97; N, 12.7%

Found: C, 54.23; H, 3.97; N, 12.69%

The thiomethyl compounds of Example XVIII are oxidized to the corresponding sulfinylmethyl compounds by this procedure.

EXAMPLE XXIII

Repetition of the procedures of Examples V-A, XXI A-C and XXII, but using the appropriate chloro substituted 2-nitrobenzaldehydes as reactants, affords the following compounds: ##STR9##R₂ R₃ R₄ R₅______________________________________H H SOCH₃ HSOCH₃ H H HH H H SOCH₃H SOCH₃ SOCH₃ HSOCH₃ H H SOCH₃H SOC₇ H₇ H HH SOC₇ H₇ C₂ H₅ HH H SOC₇ H₇ HSOC₇ H₇ H H SOC₇ H₇______________________________________

EXAMPLE XXIV 2-Methyl-7-Hydroxy-8-Methoxypyrimido[4,5-b]Quinoline-4(3H)One A. 7-Benzyl ether of title compound: A. 7-Benzyl ether of title compound:

A mixture of 6-benzyloxy-7-methoxy-2-amino-quinoline-3-carboxamide (1.50 g.), concentrated sulfuric acid (0.05 ml.) and acetic anhydride (20 ml.) is heated at 85°C. for 15 minutes. The mixture is then cooled and ice chips added to the reaction mixture. The yellow solid which precipitates is filtered, washed with water, and then with isopropyl alcohol-ether (1:1) and air-dried. Yield = 1.60 g. (99%); m.p. 202°-203°C. (dec.).

B. Debenzylation of 7-benzyl ether.

The 7-benzyl ether from preparation A above is treated with trifluoroacetic acid according to the procedure of Example XV to give the title compound in 95% yield; m.p. 135°C. (dec.).

EXAMPLE XXV 2-Methyl-7,8-Dimethoxypyrimido[4,5-b]Quinolin-4(3H)-One

Concentrated sulfuric acid (1.5 ml.) is added to a slurry of 6,7-dimethoxy-2-aminoquinoline-3-carboxamide (5.0 g., 0.02 mole) and acetic anhydride (40 ml.). The slurry dissolves to give a dark orange solution from which a heavy yellow precipitate separates. Acetic anhydride (10 ml.) is added to facilitate stirring and heating continued for an additional 75 minutes. The mixture is cooled, water (50 ml.) added and the resulting solution made alkaline with 5N sodium hydroxide (225 ml.). It is chilled, the precipitate collected by filtration and air-dried. The yellow solid is recrystallized from ethanol. Yield = 2.37 g., M.P. 300°-302° (dec.).

In like manner, the following compounds are prepared from appropriate reactants: ##STR10##R₁ R₂ R₃ R₄ R₅______________________________________H H OCH₃ OC₂ H₅ HH H F H HH H H F HH H Cl H HH H H OCH₃ HH H H H OCH₃H Cl H H HH OCH₃ H H OCH₃H H --O--CH₂ --O-- HH OCH₃ --O--CH₂ --O-- HH H t-C₄ H₉ H HH H --O--CH₂ --CH₂ --O-- H H OCH₃ OCH₃ OCH₃ HH H OC₂ H₅ OC₂ H₅ HH H CH₃ CH₃ HH Br H H HC₆ H₅ H H H HC₆ H₅ H OCH₃ OCH₃ HC₆ H₅ H CH₃ CH₃ HC₆ H₅ Cl H H HC₆ H₅ H --O--CH₂ --O-- HC₆ H₅ H Cl Br ClC₆ H₅ H t-C₄ H₉ H HCH₃ H H H HCH₃ H H OCH₃ HCH₃ H OCH₃ OC₂ H₅ HCH₃ OCH₃ H H OCH₃CH₃ H CH₃ H HCH₃ H H Cl HC₂ H₅ H H H HC₂ H₅ H OCH₃ OCH₃ HC₂ H₅ H C₂ H₅ H HC₂ H₅ Cl H H Hn-C₃ H₇ H H H Hn-C₃ H₇ H OCH₃ OCH₃ Hn-C₄ H₉ H H H Hn-C₄ H₉ H OCH₃ OCH₃ Hn-C₄ H₉ OCH₃ H H OCH₃n-C₄ H₉ H Cl H Hn-C₄ H₉ H n-C₃ H₇ n-C₃ H₇ Hn-C₄ H₉ OCH₃ --O--CH₂ --O-- Hn-C₄ H₉ H SCH₃ H Hn-C₄ H₉ H SOCH₃ H HCH₃ H SCH₃ H HCH₃ H SOCH₃ H HH H SCH₃ H HH H SOCH₃ H HH H SCH₃ SCH₃ HH H SOCH₃ SOCH₃ HC₆ H₅ H SCH₃ H HC₆ H₅ H SOCH₃ H HC₆ H₅ H OCH₃ OC₇ H₇ HCH₃ CH₃ CH₃ CH₃ OCH₃CH₃ H OCH₃ H HH H OCH₃ OC₇ H₇ HH H OC₇ H₇ OCH₃ HH H SC₇ H₇ H HH H H OC₇ H₇ OCH₃H H H OC₇ H₇ HH OC₇ H₇ OCH₃ H HH OC₇ H₇ H H HH OC₇ H₇ Br H BrH H OCH₃ OC₇ H₇ BrH C₂ H₅ H OC₇ H₇ HH C₂ H₅ OC₇ H₇ H HH H SC₇ H₇ OC₂ H₅ HH H OC₂ H₅ SC₇ H₇ HH H Br SC₇ H₇ HH H Cl OC₇ H₇ HH H F OC₇ H₇ HC₆ H₅ OC₇ H₇ OCH₃ OCH₃ HC₆ H₅ H OCH₃ OC₇ H₇ HC₆ H₅ H OC₇ H₇ OCH₃ HC₆ H₅ H SC₇ H₇ H HC₆ H₅ H H OC₇ H₇ OCH₃C₆ H₅ H Cl OC₇ H₇ HCH₃ H OC₇ H₇ OCH₃ HCH₃ H SC₇ H₇ OC₂ H₅ HCH₃ H F OC₇ H₇ HC₂ H₅ H OC₇ H₇ H Hn-C₃ H₇ H OC₇ H₇ OCH₃ Hn-C₄ H₉ H OC₇ H₇ OCH₃ Hn-C₄ H₇ C₂ H₅ SC₇ H₇ H Hn-C₄ H₉ OC₇ H₇ OCH₃ OCH₃ H______________________________________

The benzyl ethers and benzylthio ethers enumerated above are debenzylated by treatment with trifluoroacetic acid as is described in Example XV.

EXAMPLE XXVI 2-Ethyl-7,8-Dimethoxypyrimidin[4,5-b]quinolin-4(3H)-One

To a stirred mixture of 6,7-dimethoxy-2-aminoquinoline-3-carboxamide (500 mg., 0.002 mole) in propionic anhydride (10 ml.) at 60°C. is added concentrated sulfuric acid (0.5 ml.). The reaction mixture is stirred for one hour and is then cooled to room temperature and added to water (25 ml.). The aqueous mixture is stirred and made basic with 6N sodium hydroxide. It is stirred overnight and then acidified to pH 5.0 with 10% hydrochloric acid. The yellow precipitate which forms is filtered off and recrystallized from ethanol. Yield = 283 mg., M.P. 290°-291°C. (dec.). A second crop of product separates on standing. The two crops are combined and recrystallized from chloroform: ethanol (1:1). Yield = 225 mg. (39%); M.P. 293°C.

The following compounds are prepared in like manner from appropriate reactants. ##STR11##R₁ R₂ R₃ R₄ R₅______________________________________H H H H HH OCH₃ H H HH H OCH₃ H HH H H OC₂ H₅ HH H OCH₃ OC₂ H₅ HH H OC₂ H₅ OC₂ H₅ HH H OC₂ H₅ OCH₃ HH H OC₂ H₅ O--(n-C₄ H₉) HH H OC₂ H₅ OC₇ H₇ HH H H OCH₃ OCH₃H H H OCH₃ HH OCH₃ H H OCH₃H H OCH₃ H OCH₃H H SCH₃ H HH H SOCH₃ H HH H OCH₃ OCH₃ OCH₃H H H F HH H F H HH H Cl H HH Cl H H HH H --O--CH₂ --O-- HH H --O--CH₂ CH₂ --O-- HH OCH₃ --O--CH₂ --O-- HH H CH₃ CH₃ HH H C₂ H₅ H HH H t-C₄ H₉ H HH H CH₃ H HH H n-C₃ H₇ n-C₃ H₇ HH Br H H HH H Cl Br ClH H H I HC₆ H₅ H H H HC₆ H₅ H OCH₃ OCH₃ HC₆ H₅ H CH₃ CH₃ HC₆ H₅ Cl H H HC₆ H₅ H --O--CH₂ --O-- HC₆ H₅ H t-C₄ H₉ H HCH₃ H OCH₃ OC.sub. 2 H₅ HCH₃ H CH₃ H HCH₃ H H Cl HC₂ H₅ H H H HC₂ H₅ H OCH₃ OCH₃ HC₂ H₅ H n-C₃ H₇ H HC₂ H₅ --O--CH₂ --O-- OCH₃ HC₂ H₅ Br H H Hn-C₃ H₇ H --O--CH₂ CH₂ --O-- Hn-C₃ H₇ H H OC₂ H₅ Hn-C₄ H₉ H OCH₃ OCH₃ Hn-C₄ H₉ H H i-C₃ H₇ Hn-C₄ H₉ H SCH₃ H Hn-C₄ H₉ H SOCH₃ H HCH₃ H --O-(n-C.sub. 4 H₉) --O-(n-C₄ H₉) HC₆ H₅ H SCH₃ SCH₃ HC₆ H₅ H SOCH₃ SOCH₃ HH H OCH₃ OC₇ H₇ HH H OC.sub. 7 H₇ OCH₃ HH H OC₇ H₇ H HH H F OC₇ H₇ HH H Cl OC₇ H₇ HH C₂ H₅ H OC₇ H₇ HH OC₇ H₇ OCH₃ OCH₃ HC₆ H₅ H OC₇ H₇ OCH₃ HC₆ H₅ H OC₇ H₇ H HC₆ H₅ OC₇ H₇ Br H BrCH₃ H OC₇ H₇ OCH₃ HCH₃ H OCH₃ OC₇ H₇ HCH₃ H OC₇ H₇ OC₂ H₅ HCH₃ H OCH₃ OC₇ H₇ BrC₂ H₅ H F OC₇ H₇ HC₂ H₅ H OC₇ H₇ OCH₃ HC₂ H₅ OC₇ H₇ OCH₃ H Hn-C₃ H₇ H OC₇ H₇ OC₂ H₅ Hi-C₃ H₇ H OC₇ H₇ OC₂ H₅ Hn-C₄ H₉ H OC₇ H₇ H Hn-C₄ H₉ C₂ H₅ OC₇ H₇ H Hn-C₄ H₉ H OC₇ H₇ OCH₃ H______________________________________

Debenzylation of the benzyl ethers cited above by reaction with trifluoroacetic acid according to the procedure of Example XV affords the corresponding hydroxy compounds.

EXAMPLE XXVII 2-Acetyl-7,8-Dimethoxypyrimido[4,5-b]quinoline-4(3H)-One

To a solution of selenium dioxide (24.5 mg., 2.2 millimoles) in dioxane water (11 ml. of 10:1) is added 2-ethyl-7,8-dimethoxypyrimido) [4,5-b]quinolin-4(3H)-one (125 mg., 4.4 millimoles). The mixture is heated to reflux for 48 hours after which more selenium dioxide (24.5 mg.) is added and refluxing continued for an additional 24 hours. The mixture is cooled, the selenium filtered off and the filtrate concentrated to dryness. The residue is taken up in ethanol/chloroform (1:99) and chromatographed on a column of silica using the same solvent as eluant (250 ml.) followed by ethanol/chloroform (2:98). Concentration of the second eluate (625 ml.) gives a yellow solid (23 mg.); M.P. 300°C. [dec.]

Similarly, the remaining 2-ethyl derivatives of Example XXVI are oxidized to their corresponding 2-acetyl derivatives.

EXAMPLE XXVIII Ethyl 5-Phenylpyrimido[4,5-b]Quinolin-4(3H)-One-2-Carboxylate

A mixture of 2-amino-4-phenylquinoline-3-carboxamide (263 mg., 1.0 millimole), diethyl oxalate (5 ml.) acetic acid (5 ml.) is heated to reflux overnight. It is then cooled, diluted with ether (50 ml.) and the brown solid which forms filtered off. The filtrate is evaporated in vacuo and the oily residue triturated with saturated aqueous sodium bicarbonate solution. The resulting solid (110 mg.) is recovered by filtration and recrystallized from hot ethyl acetate (8 ml.). A small amount of insoluble material is filtered off from the hot solution. Upon cooling, the title product separates as crystals (25 mg.). M.P. = 255° C. (dec.).

Analysis: Calcd. for C₂₀ H₁₅ N₃ O₃ :C, 69.55; H, 4.38; N, 12.17%

Found*: C, 69.94; H, 4.31; N, 12.17%.

In like manner, the 2-amino-4-substituted-quinoline-3-carboxamides of Preparation D are converted to ethyl 5-substituted-pyrimido[4,5-b]quinolin-4(3H)-one-2-carboxylates of the formula ##SPC3##

wherein the R variables have the values given in Preparation D.

The esters thus obtained are transformed to the corresponding amide and hydroxamic acid derivatives by the procedures of Examples VII and XIV, respectively.

EXAMPLE XXIX Ethyl 3-Methyl-7,8-Dimethoxypyrimido[4,5-b]quinolin-4(3H)-One-2-Carboxylate

Sodium hydride (470 mg. of 50% in oil, 0.011 mole) is added to a slurry of ethyl 7,8-dimethoxypyrimido[4,5-b]quinolin-4(3H)-one-2-carboxylate (3.3 g., 0.01 mole) in N,N-dimethylformamide (75ml.). The mixture is stirred and heated on a steambath for ten minutes and then at room temperature for a half hour. It is cooled in an ice-bath and iodomethane (2.1 g., 0.015 mole) added dropwise over a 30 minute period. Following completion of addition, the mixture is stirred for an additional 15 minutes in an ice-bath and then for a half-hour at room temperature. The reaction mixture is poured into ice-water (200 ml.) and the resulting solid filtered off, air-dried, and recrystallized from ethanol to give 2.1 g. of yellow crystals (61.2%); m.p. 211.5°C. (dec.)

Analysis: Calcd. for C₁₇ H₁₇ N₃ O₅ : C, 59.47; H, 4.99; N, 12.24%

Found: C, 59.55; H, 5.02; N, 12.45%

In like manner, but using the appropriate compound of formula I (Y=H) and the appropriate alkyl iodide, the products of Examples I and III-XXVIII are converted to their corresponding 3-methyl, 3-ethyl, 3-n-propyl, 3-isopropyl and 3-n-butyl derivatives.

Compounds wherein one of R₂, R₃, R₄, or R₅ is thiol or hydroxy are obtained by debenzylation of the corresponding benzylthio ethers or benzylethers by reaction with trifluoroacetic acid according to the procedure of Example XV.

EXAMPLE XXX Ethyl 3-Carbethoxymethyl-7,8-Dimethoxypyrimido[4,5-b]quinolin-4(3H)-One-2-Carboxylate

To a slurry of ethyl 7,8-dimethoxypyrimido[4,5-b]quinolin-4-(3H)-one-2-carboxylate (1.6 g., 5.0 millimoles) in N,N-dimethylformamide (30 ml.) is added sodium hydride (235 mg., 5.5 millimoles of 56% in oil). The slurry is heated on a steambath for ten minutes and then cooled to room temperature.

Ethyl bromoacetate (968 mg., 5.8 millimoles) is added and the reaction mixture heated on a steambath for one hour. After standing overnight at room temperature the mixture is diluted with water (75 ml.) and the resulting yellow solid filtered off (1.4g.). It is purified by chromatography on a column of silica gel using chloroform as solvent and eluant. The eluate (300 ml.) is evaporated to give 1.2g. of yellow solid. It is recrystallized from hot ethanol (50 ml.) containing sufficient chloroform to achieve solution. Yield = 900 mg. of fluffy yellow crystals (43.4%); m.p. 198- 199°C. (dec.)

Analysis: Calcd. for C₂₀ H₂₁ N₃ O₇ : C, 57.83; H, 5.10; N, 10.12%

Found: C, 59.67; H, 4.99; N, 9.90%.

EXAMPLE XXXI Ethyl 3-(3-Carbethoxypropyl)-7,8-Dimethoxypyrimido[4,5-b]quinolin-4(3H)-One-2-Carboxylate

The procedure of Example XXX is repeated but using ethyl 4-bromobutyrate (1.1 g., 5.8 millimole) in place of ethyl bromoacetate. The solid obtained upon diluting the reaction mixture with water is filtered off, dried and recrystallized from benzene: hexane (40 ml. of 1:1) to give 615 mg. (29.1%) of the title compound as fluffy yellow crystals; M.P. 144°-146°C.

Analysis: Calcd. for C₂₂ H₂₅ N₃ O₇ : C, 59.58; H, 5.68; N, 9.48%

Found: C, 59.84; H, 5.98; H, 9.61%

EXAMPLE XXXII

Pyrimido [4,5-b]quinolin-4(3H)-one compounds of Examples I-XXVIII are alkylated substantially according to the procedure of Example XXXI but using the appropriate alkyl bromoalkanoate in place of ethyl 4-bromo butyrate. The products are recrystallized from suitable solvents such as ethanol or benzene: hexane (1:1). ##STR12##Y R R₁ R₂ R₃ R₄ R₅__________________________________________________________________________CH₂ COOCH₃ COOC₂ H₅ H H H H HCH₂ COO--n-C₄ H₉ COOC₂ H₅ H H H H HCH₂ COOC₂ H₅ COO--n-C₄ H₉ H H OCH₃ OCH₃ HCH₂ COO--n-C₃ H₇ COOC₂ H₅ H H OCH H OCH₃CH₂ CH₂ COOCH₃ COOC₂ H₅ H OCH₃ H H OCH₃CH₂ CH₂ COO--n-C₃ H₇ COOC₂ H₅ H H OCH₃ OCH₃ H(CH₂)₄ COOC₂ H₅ COOC₂ H₅ H H OCH₃ OCH₃ OCH₃(CH₂)₄ COO--n-C₄ H.sub. 9 COOC₂ H₅ H H OC₂ H₅ O--n-C₄ H₉ HCH₂ COOC₂ H₅ COOC₂ H₅ H H OC₂ H₅ OC₇ H₇ H(CH₂)₃ COOCH₃ COOC₂ H₅ H OC₂ H₅ OC₂ H₅ H HCH₂ COOC₂ H₅ COOC₂ H₅ H H --OCH₂ CH₂ O-- HCH₂ COOCH₃ COOC₂ H₅ H H F H HCH₂ CH₂ COOC₂ H₅ COOC₂ H₅ H H H F HCH₂ COO--i-C₃ H₇ COOC₂ H₅ H H Cl H HCH₂ CH₂ COOCH₃ COOC₂ H₅ H H --OCH₂ --O-- HCH₂ COOCH₃ COO--n-C₄ H₉ H H H H HCH₂ CH₂ COOCH₃ COOC₂ H₅ H Cl H H HCH₂ COO--i-C₃ H₇ COOC₂ H₅ H H H OCH₃ OCH₃CH₂ COOC₂ H₅ COOCH₂ CH₂ OH H H OCH₃ OCH₃ H(CH₂)₃ COOCH₃ COOC₂ H₅ H H O--n-C₄ H₉ O--n-C₄ H₉ HCH₂ CH₂ COOCH₃ COOC₂ H₅ H OCH₃ --O--CH₂ --O-- HCH₂ COOC₂ H₅ COOC₂ H₅ H H Br H Br(CH₂)₄ COOC₂ H₅ COOC₂ H₅ Cl H H Cl(CH₂)₃ COO--n-C₃ H₇ COO--i-C₃ H₇ H H CH₃ H HCH₂ CH₂ COOC₂ H₅ COOC₂ H₅ H H H CH₃ HCH₂ COOC₂ H₅ COOC₂ H₅ H H t-C₄ H₉ H HCH₂ COOCH₃ COOCH₃ H CH₃ H CH₃ HCH₂ COOC₂ H₅ COOC₂ H₅ H H n-C₃ H₇ H HCH₂ COOC₂ H₅ COOC₂ H₅ H H SCH₃ H HCH₂ COOC₂ H₅ COOC₂ H₅ C₆ H₅ H H H HCH₂ COOC₂ H₅ COOC₂ H₅ C₆ H₅ H OCH₃ OCH₃ H(CH₂)₃ COOCH₃ COOC₂ H₅ CH₃ H OCH₃ OCH₃ H(CH₂)₄ COOC₂ H₅ COOC₂ H₅ CH₃ H H OC₂ H₅ OC₂ H₅CH₂ COO--n-C₄ H₉ COOC₂ H₅ CH₃ H CH₃ CH₃ HCH₂ CH₂ COOC₂ H₅ n-C₃ H₇ H Cl H HCH₂ COO--n-C₃ H₇ COOC₂ H₅ CH₃ H Cl CH₃ HCH₂ COOn-C₄ H₉ COOC₂ H₅ n-C₄ H₉ H H H H(CH₂)₃ COOC₂ H₅ COOC₂ H₅ C₂ H₅ H Br H HCH₂ COOCH₃ COOC₂ H₅ C₆ H₅ H F H HCH₂ COOC₂ H₅ COOC₂ H₅ CH₃ H --OCH₂ CH₂ --O-- HCH₂ COOC₂ H₅ COOC₂ H₅ C₆ H₅ H --O--CH₂ --O-- HCH₂ COOC₂ H₅ COOCH₂ CH₂ OH H H OCH₃ OCH₃ HCH₃ COOC₂ H₅ COO(CH₂)₃ OH H H OC₂ H₅ OCH₃ HCH₂ COOCH₃ COO(CH₂)₄ OH H H H HCH₂ COOCH₂ CH₂ OH COOCH₂ CH₂ OH H H OCH₃ OCH₃ HCH₂ COOC₂ H₅ CH₃ H H OCH₃ OCH₃ H(CH₂)₃ COOC₂ H₅ CH₃ H Cl H H HCH₂ COOCH₃ CH₃ H H F H H(CH₂)₂ COOC₂ H₅ CH₃ H H -- O--CH₂ --O-- HCH₂ COOC₂ H₅ CH₃ H H t-C₄ H₉ H HCH₂ COOC₂ H₅ CH₃ C₆ H₅ H SCH₃ H HCH₂ CH₂ COOCH₃ C₂ H₅ H H OCH₃ OCH.sub. HCH₂ COOC₂ H₅ C₂ H₅ CH₃ H Cl Br ClCH₂ COOC₂ H₅ C₂ H₅ n-C₄ H₉ H SOCH₃ H HCH₂ COOCH₃ COCH₃ H H OCH₃ OCH₃ H(CH₂)₂ COOCH₃ COCH₃ H Cl H H H(CH₂)₂ COOCH₃ COCH₃ C₆ H_(H) CH₃ H H(CH₂)₃ COOC₂ H₅ COCH₃ C₂ H₅ H H H HCH₂ COOCH₃ COOC₂ H₅ H H OC₇ H₇ OCH₃ H(CH₂)₂ COOC₂ H₅ COO--n-C₄ H₉ H H OC₇ H₇ OCH₃ HCH₂ COO--n-C₄ H₉ COOC₂ H₅ H H OC₇ H₇ H H(CH₂)₃ COOC₂ H₅ COOC₂ H₅ H H OCH₃ OC₇ H₇ HCH₂ COOC₂ H₅ COOCH₃ H H OC₇ H₇ OC₇ H₇ H(CH₂)₄ COOCH₃ COOC₂ H₅ H OC₇ H₇ OCH₃ OCH₃ H(CH₂)₂ COO--n-C₄ H₉ COO--n-C₄ H₉ H H H H OC₇ H₇CH₂ COO--n-C₃ H₇ COO--n-C₃ H₇ H C₂ H₅ OC₇ H₇ H HCH₂ COOC₂ H₅ COOC₂ H₅ H H Br OC₇ H₇ HCH₂ COOC₂ H₅ COOCH₃ H H Cl OC₇ H₇ HCH₂ COOCH₃ COOCH₂ CH₂ OH H H OC₇ H₇ OCH₃ HCH₂ COOCH₃ COOCH₂ CH₂ OH H H OCH₃ OC₇ H₇ H(CH₂)₂ COOC₂ H₅ COO(CH₂)₄ OH H H OC₇ H₇ H HCH₂ COO--n-C₄ H₉ COO(CH₂)₃ OH H H F OC₇ H₇ HCH₂ COOC₂ H₅ CH₃ H H OC₇ H₇ OCH₃ HCH₂ COOC₂ H₅ CH₃ H H OC₂ H₅ OC₇ H₇ HCH₂ COOC₂ H₅ CH₃ H OC₇ H₇ OCH₃ H HCH₂ COOC₂ H₅ C₂ H₅ H H OC₇ H₇ H HCH₂ COOC₂ H₅ C₂ H₅ H H OCH₃ OC₇ H₇ BrCH₂ COOC₂ H₅ C₂ H₅ H H Br OC₇ H₇ HCH₂ COOC₂ H₅ COCH₃ H H OC₇ H₇ OCH₃ HCH₂ COOC₂ H₅ COCH₃ H C₂ H₅ H OC₇ H₇ HCH₂ COOCH₃ CH₃ C₆ H₅ H OC₇ H₇ OCH₃ H(CH₂)₃ COOC₂ H₅ C₂ H₅ C₂ H₅ OC₇ H₇ OCH₃ H H(CH₂)₂ COO--n-C₄ H₉ CH₃ n-C₄ H₉ C₂ H₅ OC₇ H₇ H HCH₂ COOC₂ H₅ CH₃ CH₃ H OC₇ H₇ OCH₃ HCH₂ COOCH₃ COOC₂ H₅ H H SC₇ H₇ H H(CH₂)₄ COOC₂ H₅ COOC₂ H₅ H H H H SC₇ H₇CH₂ COOC₂ H₅ COOC₂ H₅ H H SC₇ H₇ C₂ H₅ H(CH₂)₂ COO--n-C₄ H₉ COOC₂ H₅ H H H SC₇ H₇ HCH₂ COOCH₃ COOC₂ H₅ H SC₇ H₇ H H SC₇ H₇CH₂ COOCH₃ COOC₂ H₅ H SOC₇ H₇ H H H(CH₂)₃ COO--i-C₃ H₇ COOC₂ H₅ H H SOC₇ H₇ C₂ H₅ HCH₂ CH₂ COOC₂ H₅ COOC₂ H₅ H H SOC₇ H₇ H HCH₂ COOCH₃ COOC₂ H₅ H H CH₃ COO OCH₃ H(CH₂)₄ COOC₂ H₅ COOC₂ H₅ H H OCH₃ C₆ H₅ COO H__________________________________________________________________________

The benzyl ethers and benzylthio ethers tabulated above are converted to the corresponding hydroxy and thiol compounds by reaction with trifluoroacetic acid according to the procedure of Example XV.

EXAMPLE XXXIII Ethyl 3-Acetoxyethyl-7,8-Dimethoxypyrimidin[4,5-b]quinolin-4(3H)-One-2-Carboxylate

The procedure of Example XXX is repeated but using bromoethyl acetate (969 mg., 5.8 millimoles) as alkylating agent in place of ethyl bromoacetate. The solid product which separates upon dilution of the reaction mixture with water is filtered off, dried and recrystallized from ethanol (20 ml.) containing sufficient acetonitrile to achieve solution. Yield = 550 mg. (26.5%); M.P. 195°-196°C. (dec.)

Analysis: Calcd. C₂₀ H₂₁ N₃ O₇ : C, 57.83; H, 5.10; N, 10.12%

Found C, 57-95; H, 5.14; N, 10.31%

In like manner, the following compounds are prepared from the appropriate pyrimidin[4,5-b]quinolin-4(3H)-one compounds of Examples I-XXVIII and the appropriate bromoalkanoate or bromoalkyl benzoate: ##STR13##Y R R₁ R₂ R₃ R₄ R₅__________________________________________________________________________CH₂ CH₂ OCOCH₃ COOC₂ H₅ H H OC₂ H₅ H HCH₂ CH₂ OCOC₂ H₅ COOC₂ H₅ H H H OCH₃ HCH₂ CH₂ OCOCH₃ COOC₂ H₅ H H --O--CH₂ --O-- H(CH₂)₃ OCOCH₃ COOC₂ H₅ H H OC₂ H₅ OC₇ H₇ H(CH₂)₄ OCOC₂ H₅ COOC₂ H₅ H OCH₃ H H OCH₃CH₂ CH₂ OCOC₆ H₅ COOC₂ H₅ H H OCH₃ OCH₃ HCH₂ CH₂ OCOC₃ H₇ H H F H H(CH₂)₃ OCOC₂ H₅ COOC₂ H₅ C₂ H₅ H Hl Cl HCH₂ CH₂ OCOCH₃ COOCH₂ CH₂ OH H H OCH₃ OCH₃ HCH₂ CH₂ OCOC₂ H₅ COOC₂ H₅ H H SCH₃ H HCH₂ CH₂ OCOC₂ H₅ COOC₂ H₅ H H SOCH₃ H H(CH₂)₄ OCOC₄ H₉ COOC₂ H₅ C₆ H₅ H H H HCH₂ CH₂ OCOCH₃ COO--n-C₄ H₉ H H OCH₃ OCH₃ HCH₂ CH₂ OCOC₂ H₅ COOC₂ H₅ H H OCH₃ H OCH₃(CH₂)₃ OCOCH₃ COOC₂ H₅ H H OC₂ H₅ O--n-C₄ H₉ HCH₂ CH₂ OCOC₃ H₇ COOC₂ H₅ H H --OCH₂ CH₂ O-- H(CH₂)₄ OCOCH₃ COOC₂ H₅ H H H F HCH₂ CH₂ OCOC₂ H₅ COOC₂ H₅ H Cl H H HCH₂ CH₂ OCOC₄ H₉ COO--n-C₄ H₉ H H H H HCH₂ CH₂ OCOCH₃ COOC₂ H₅ H H OCH₃ OCH₃ OCH₃(CH₂)₃ OCOC₂ H₅ COOC₂ H₅ H Br H H HCH₂ CH₂ OCOCH₃ COOCH₃ H CH₃ H CH₃ HCH₂ CH₂ OCOC₃ H₇ COOC₂ H₅ H H t-C₄ H₉ H H(CH₂)₄ OCOCH₃ COOC₂ H₅ H H n-C₃ H₇ n-C₃ H₇ HCH₂ CH₂ OCOC₂ H₅ CH₃ H H OCH₃ OCH₃ HCH₂ CH₂ OCOCH₃ CH₃ CH₃ H OCH₃ OC₂ H₅ H(CH₂)₃ OCOCH₃ CH₃ n-C₄ H₉ H H H HCH₂ CH₂ OCOC₂ H₅ CH₃ H H SCH₃ SCH₃ HCH₂ CH₂ OCOC₂ H₅ CH₃ H H SOCH₃ SOCH₃ HCH₂ CH₂ OCOC₂ H₅ CH₃ C₂ H₅ Cl H H HCH₂ CH₂ OCOCH₃ CH₃ C₆ H₅ H OCH₃ OCH₃ H(CH₂)₄ OCOCH₄ CH₃ C₆ H₅ H SCH₃ H H(CH₂)₃ OCOC₂ H₅ C₂ H₅ H H H H H(CH₂)₃ OCOC₄ H₉ C₂ H₅ H H OCH₃ OCH₃ HCH₂ CH₂ OCOCH₃ C₂ H₅ H H Cl H HCH₂ CH₂ OCOC₂ H₅ C₂ H₅ H H OC₂ H₅ OC₇ H₇ H(CH₂)₃ OCOC₂ H₅ C₂ H₅ H H OC₂ H₅ O--n-C₄ H₉ H(CH₂)₃ OCOC₂ H₅ C₂ H₅ H H OC₂ H₅ O--n-C₄ H₉ H(CH₂)₃ OCOC₂ H₅ C₂ H₅ H OCH₃ --O--CH₂ --O-- H CH₂ CH₂ OCOC₂ H₅ C₂ H₅ C₂ H₅ H SCH₃ SCH₃ HCH₂ CH₂ OCOCH₃ C₂ H₅ n-C₄ H₉ H H i-C₃ H₇ HCH₂ CH₂ OCOC₂ H₅ C₂ H₅ CH₃ H O--n-C₄ H₉ O--n-C₄ H₉ HCh₂ CH₂ OCOCH₃ C₂ H₅ C₆ H₅ H SOCH₃ SOCH₃ H(CH₂)₃ OCOCH₃ COCH₃ H H OCH₃ OCH₃ H(CH₂)₃ OCOCH₃ COCH₃ H H --OCH₂ CH₂ O-- HCH₂ CH₂ OCOCH₃ COCH₃ H H CH₃ CH₃ HCH₂ CH₂ OCOCH₃ COCH₃ CH₃ H H Cl HCH₂ CH₂ OCOCH₃ COCH₃ C₂ H₅ Br H H HCH₂ CH₂ OCOCH₃ COCH₃ n-C₄ H₉ H H i-C₃ H₇ HCH₂ CH₂ OCOCH₃ COCH₃ C₆ H₅ H SOCH₃ SOCH₃ HCH₂ CH₂ OCOC₆ H₅ COOC₂ H₅ H H OCH₃ OCH₃ H(CH₂)₄ OCOC₆ H₅ COOC₂ H₅ H H --O--CH₂ --O-- HCH₂ CH₂ OCOC₆ H₅ H H H OC₂ H₅ OC₇ H₇ H(CH₂)₃ OCOC₆ H₅ COOC₂ H₅ CH₃ H SCH₃ H HCH₂ CH₂ OCOC₆ H₅ CH₃ C₆ H₅ CH₃ CH₃ CH₃ OCH₃(CH₂)₄ OCOC₆ H₅ CH₃ CH₃ H SOCH₃ H HCH₂ CH₂ OCOC₆ H₅ C₂ H₅ H H OC₂ H₅ O--(n-C₄ H₉) HCH₂ CH₂ OCOC₆ H₅ COOC₂ H₅ H H OC₇ H₇ OCH₃ HCH₂ CH₂ OCOC₆ H₅ COO--n-C₄ H₉ H H OC₇ H₇ OCH₃ HCH₂ CH₂ OCOCH₃ COOC₂ H₅ H H OCH₃ OC₇ H₇ H(CH₂)₄ OCOC₃ H₇ COOC₂ H₅ H H OC₇ H₇ H H(CH₂)₃ OCOC₂ H₅ COOC₂ H₅ H OC₇ H₇ OCH₃ H HCH₂ CH₂ OCOC₂ H₅ COOC₂ H₅ H H F OC₇ H₇ HCH₂ CH₂ OCOCH₃ CH₃ H H OC₇ H₇ OCH₃ H(CH₂)₃ OCOC₆ H₅ CH₃ C₆ H₅ H OCH₃ OCH₃ HCH₂ CH₂ OCOCH₃ CH₃ n-C₄ H₉ C₂ H₅ OC₇ H₇ H HCH₂ CH₂ OCOC₃ H₇ C₂ H₅ H H OC₇ H₇ OC₇ H₇ HCH₂ CH₂ OCOCH₃ C₂ H₅ CH₃ H OCH₃ OC₇ H₇ BrCH₂ CH₂ OCOCH₃ COCH₃ H H OC₇ H₇ OCH₃ HCH₂ CH₂ OCOC₆ H₅ COOCH₂ CH₂ OH H H OC₇ H₇ OCH₃ HCH₂ CH₂ OCOCH₃ COOC₂ H₅ H H SC₇ H₇ H H(CH₂)₄ OCOC₂ H₅ COOC₂ H₅ H H SC₇ H₇ C₂ H₅ HCH₂ CH₂ OCOC₆ H₅ COOC₂ H₅ H H H SC₇ H₇ HCH₂ CH₂ OCOC₃ H₇ COOC₂ H₅ H H SOC₇ H₇ H HCH₂ CH₂ OCOC₆ H₅ COOC₂ H₅ H H H SOC₇ H₇ HCH₂ CH₂ OCOCH₃ COO--n-C₄ H₉ H H CH₃ COO OCH₃ H(CH₂)₄ OCOC₂ H₅ COOC₂ H₅ H H C₃ H₇ COO OCH₃ HCH₂ CH₂ OCOC₆ H₅ COOCH₃ H HCOO OCH₃ H H(CH₂)₃ OCOCH₃ COOC₂ H₅ H H H C₂ H₅ COS HCH₂ CH₂ OCOC₃ H₇ COOC₂ H₅ H H C₆ H₅ COO OCH₃ HCH₂ CH₂ OCOCH₃ COOC₂ H₅ H H CH₃ COO H H(CH₂)₄ OCOC₂ H₅ COOC₂ H₅ H H C₆ H₅ COO H H__________________________________________________________________________

EXAMPLE XXXIV Ethyl Benzo[g]Quinazolin-4(3H)-One-2-Carboxylate A. Benzo-isatoic Anhydride

Anthranilic acid (25.0 g., 0.133 mole) is dissolved, warming if necessary, in a mixture of water (50 ml.), concentrated hydrochloric acid (50 ml.) and dioxane (100 ml.). Phosgene is passed into the solution with good stirring at such a rate that bubbles of the gas escape slowly into an ammonia scrubber attached to the reaction flask. The temperature is held below 50°C. by regulating the rate of introduction of the phosgene. After passing phosgene into the mixture for four hours, the residual phosgene is blown out by passing air through the mixture. The mixture is cooled and the product filtered off, washed with cold water and dried to give 26.46 g. (93.5%) of the anhydride. M.P. 390°C. (dec.).

B. 2-Aminonaphthalene-3-Carboxamide

Ammonia is intermittently bubbled into a suspension of benzoisatoic anhydride (5.0 g., 2.25 millimoles) and ethanol (80 ml.) for a period of two days. The bright green solid which formed is filtered from the reaction mixture, dried and then recrystallized from ethanol. Yield = 1.95 g. (46%) of the title amide. M.P. 234°-235°C. (m/e - 186).

C. Ethyl benzo[g]quinazolin-4(3H)-one-2-carboxylate

Diethyl oxalate (2.35 g., 16.1 millimoles), sodium methoxide (10 mg) and 2-aminonaphthalene-3-carboxamide (1.50 g., 8.05 millimoles) are mixed together and heated at reflux for 2 days. The mixture is then cooled in an ice bath and the brown precipitate recovered by filtration. It is recrystallized from ethanol to give 1.76 g. (79% yield) of the desired ester. M.P. 240°-242°C.

Analysis: Calcd. for C₁₅ H₁₂ N₂ O₃ : C, 67.15; H, 4.51; N, 10.44%.

Found: C, 65.44; H, 4.50; N, 10.17%.

D. The ester is hydrolyzed to the disodium salt as follows:

The ester (1.05 g., 3.92 millimoles) is added to 15% aqueous sodium hydroxide (20 ml.) and the mixture stirred for 2 days. It is then acidified at room temperature with 10% hydrochloric acid to pH 2 and evaporated to dryness under reduced pressure. The residue is treated with methanol-water (1:1, 50 ml.) and the suspension filtered to give 0.70 g. of yellow crystals. The crystals are added to a saturated solution of aqueous sodium bicarbonate (35 ml.) and water (20 ml.). The mixture is stirred for a half-hour and the yellow solid filtered off and dried. Yield = 0.641 g. (54.7%). M.P. 340° C. (dec.).

Analysis: Calcd. for C₁₃ H₆ N₂ O₃.2Na.H₂ O: C, 51.31; H, 3.28; N, 9.06; Na, 14.10%

Found: C, 51.34; H, 3.05; N, 9.24; Na, 11.14%.

E. Repetition of this hydrolysis procedure but using potassium bicarbonate or ammonium bicarbonate in place of sodium bicarbonate affords the dipotassium salt and the diammonium salt. EXAMPLE XXXV Alkyl Benzo[g]Quinazolin-4(3H)-One-2-Carboxylates

The following alkyl benzo[g]quinazolin-4(3H)-one-2-carboxylates are prepared from the appropriate reactants by the procedure of Example XXXIV. ##STR14##R⁰ R₂ R₃ R₄______________________________________OC₂ H₅ H H OC₂ H₅OCH₃ H OCH₃ OCH₃O-- n-C₄ H₉ H OCH₃ OCH₃OC₂ H₅ H --O--CH₂ --O--OC₂ H₅ H H O-- n-C₄ H₉OC₂ H₅ H O-- n-C₃ H₇ O-- n-C₃ H₇OCH₃ H CH₃ HO--i-C.sub. 3 H₇ H H CH₃OC₂ H₅ CH₃ H HOC₂ H₅ CH₃ CH₃ HOC₂ H₅ H CH₃ CH₃O-- n-C₃ H₇ H H C₂ H₅O-- n-C₃ H₇ H H n-C₃ H₇O-- n-C₄ H₉ H H i-C.sub. 3 H₇OCH₃ H n-C₃ H₇ HOCH₃ H n-C₃ H₇ n-C₃ H₇OC₂ H₅ H t-C₄ H₉ HOC₂ H₅ CH₃ H CH₃OCH₃ H H BrOCH₃ H Br HOCH₃ Br H HOC₂ H₅ Cl H HOC₂ H₅ H Cl HOC₂ H₅ H H ClO-- n-C₄ H₉ H I HO-- n-C₄ H₉ I H HOC₂ H₅ H F HOC₂ H₅ H H FOC₂ H₅ SCH₃ H HOCH₃ H SCH₃ HO-- n-C₄ H₉ H H SCH₃OCH₃ H SC₇ H₇ HO-- n-C₃ H₇ SC₇ H₇ H HOC₂ H₅ H H SC₇ H₇OCH₃ H OC₂ H₅ HOC₂ H₅ H O-- n-C₄ H.sub. 9 HOC₂ H₅ H OC₇ H₇ HOC₂ H₅ OC₇ H₇ H H______________________________________

The methyl and ethyl esters in the above tabulation are converted to the corresponding n-butyl esters by the transesterification procedure of Example VIII, and to the acids, the disodium, diammonium and dipotassium salts according to the hydrolysis procedure of Example XXXIV-D.

The thioethers are converted to the corresponding sulfinyl derivatives by oxidation with hydrogen peroxide according to the procedure of Example XXII.

Benzyl ethers and benzylthio ethers are debenzylated by reaction with trifluoroacetic acid as described in Example XV to the corresponding hydroxy and thiol compounds.

EXAMPLE XXXVI

The hydroxy compounds of Example XXXV are converted to corresponding acyl derivatives by the procedure of Example XIX. The following are thus prepared: ##STR15##R^(o) R₂ R₃ R₄______________________________________OCH₃ H CH₃ COO HO-- n-C₃ H₇ CH₃ COO H HOCH₃ H C₆ H₅ COO HOC₂ H₅ H C₆ H₅ COO HOC₂ H₅ HCOO H HO-- n-C₄ H₉ CH₃ COO H HOCH₃ C₃ H₇ COO H H______________________________________

EXAMPLE XXXVII Benzo[g]Quinazolin-4(3H)-One-2-Carboxamides

Following the procedure of Example IX but using the appropriate 2-aminonaphthalene-3-carboxamide in place of 2-aminoquinolin-3-carboxamide, the amides corresponding to the ester products of Examples XXXIV and XXXV are prepared. The compounds have the formula: ##SPC4##

EXAMPLE XXXVIII Benzo[g]Quinazolin-4(3H)-One-2-Hydroxamic Acids

The ester products of Example XXXIV-XXXVI are converted to the corresponding hydroxamic acids by the procedure of Example XIV. The compounds have the formula: ##SPC5##

EXAMPLE XXXIX Ethyl Pyrido[2,3-d]Pyrimidin-4(3H)-One-2-Carboxylate

A mixture of diethyl oxalate (530 mg., 3.65 millimoles) and 2-aminonicotinamide (500 mg., 3.65 millimoles is heated to reflux overnight. The mixture is then cooled in an ice bath and the yellow solid which precipitated filtered off and recrystallized from benzene/hexane (1:1). Yield = 196 mg. (30%). M.P. 190°-192° C.

Analysis: Calcd. for C₁₀ H₉ N₃ O₃ : C, 54.79; H, 4.13; N, 19.17%.

Found: C, 54.88; H, 4.11; N, 19.33%

Hydrolysis of the ester according to the procedure of Example XXXIV-D affords the acid and the disodium salt.

EXAMPLE XL

Repetition of the procedure of Example XXV but using the appropriate 2-aminonicotinamide in place of 6,7-dimethoxy-2-aminoquinoline-3-carboxamide produces the following compounds: ##STR16##R₂ R₃ R₄______________________________________H H HCH₃ H CH₃H OCH₃ CH₃H OCH₃ HH t-C₄ H₉ HCl H HCH₃ Br CH₃H SCH₃ HCH₃ SCH₃ HH SOCH₃ * HCH₃ SOCH₃ * HCH₃ SC₇ H₇ HCH₃ SC₇ H₇ CH₃CH₃ SOC₇ H₇ * HCH₃ SOC₇ H₇ * CH₃CH₃ C₂ H₅ CH₃CH₃ n-C₃ H₇ CH₃CH₃ n-C₄ H₉ HCH₃ Cl CH₃H Cl HCH₃ C₂ H₅ HH Cl CH₃CH₃ OC₂ H₅ HCH₃ O-- n-C₄ H₉ CH₃CH₃ OC₇ H₇ HOC₇ H₇ H H______________________________________ *Produced by oxidation of the corresponding thioethers according to the procedure of Example XXII.

The benzyl ethers and benzylthio ethers listed above are debenzylated by treatment with trifluoroacetic acid to afford the corresponding hydroxy and thiol compounds according to the procedure of Example XV.

EXAMPLE XLI 2-Ethylpyrido[2,3-d]Pyrimidin-4(3H)-Ones

Concentrated sulfuric acid (0.5 ml.) is added to a mixture of 2-aminonicotinamide (0.24 g., 0.002 mole) in propionic anhydride (10 ml.) at 60°C. and the resulting mixture stirred for 1 hour. It is then cooled to room temperature and poured into water (25 ml.). The aqueous mixture is stirred, made basic with 6N sodium hydroxide and stirred overnight. Upon acidification to pH 5.0 with 10% hydrochloric acid the desired product precipitates. It is filtered off and dried.

The following compounds are prepared in like manner from appropriate reactants: ##STR17##R₂ R₃ R₄______________________________________H OCH₃ CH₃H OCH₃ HH Cl HH SCH₃ HH t-C₄ H₉ HCH₃ Br CH₃CH₃ SCH₃ HCH₃ Cl HCH₃ SOCH₃ * HH SOCH₃ * HH C₂ H₅ HH CH₃ HCH₃ Cl CH₃CH₃ SC₇ H₇ HCH₃ SC₇ H₇ CH₃CH₃ SOC₇ H₇ * HH Cl CH₃______________________________________ *Prepared by oxidation of the corresponding thio compound according to th procedure of Example XXII.

Debenzylation of the benzyl ethers and benzylthio ethers by treatment with trifluoroacetic acid according to the procedure of Example XV affords the corresponding hydroxy and thiol compounds.

EXAMPLE XLII 2-Acetylpyrimido[2,3-d]Pyrimidin-4(3H)-Ones

The products of Example XLI are oxidized by selenium dioxide substantially according to the procedure of Example XXVII to provide compounds of the formula: ##SPC6##

EXAMPLE XLIII

Repetition of the procedure of Example XXXV reactants produces the following compounds: ##STR18##R^(o) R₂ R₃ R₄______________________________________OC₂ H₅ H CH₃ HO-- n-C₃ H₇ CH₃ H CH₃OCH₃ H CH₃ CH₃OC₂ H₅ CH₃ H CH₃OC₂ H₅ CH₃ CH₃ CH₃OCH₃ CH₃ Br CH₃OC₂ H₅ Cl H HO-- n-C₄ H₉ H H HOCH₃ H H CH₃OC₂ H₅ H H i-C₃ H₇OCH₃ H H i-C₄ H₉OC₂ H₅ H OCH₃ CH₃O-- n-C₄ H₉ H OCH₃ HOC₂ H₅ CH₃ H HOCH₂ H₅ H n-C₃ H₇ HOCH₃ H t-C₄ H₉ HOC.sub. 2 H₅ H Cl HO-- i-C₃ H₇ H Br HOC₂ H₅ CH₃ Cl HOC₂ H₅ H Cl CH₃OCH₃ CH₃ Cl CH₃OC₂ H₅ H SCH₃ HOC₂ H₅ CH₃ SCH₃ CH₃OC₂ H₅ CH₃ SC₇ H₇ HOCH₃ CH₃ SC₇ H₇ CH₃OCH₃ CH₃ OC₇ H₇ HOC₂ H₅ OC₇ H₇ H HOC₂ H₅ CH₃ O-- n-C₄ H₉ CH₃OC₂ H₅ H SOCH₃ * HOC₂ H₅ CH₃ SOCH₃ * CH₃OC₂ H₅ CH₃ SOC₇ H₇ * HOCH₃ CH₃ SOC₇ H₇ * CH₃______________________________________ *Prepared by oxidation according to the procedure of Example XXII.

Hydrolysis of the ester by the method of Example XXIII-D produces the acid, and disodium salt derivatives. Debenzylation of benzyl ethers and benzylthio ethers in the manner of Example XV affords the corresponding hydroxy and thiol compounds.

EXAMPLE XLIV

The hydroxy and thiol compounds of Examples XL-XLIII are converted to acyloxy derivatives by the procedure of Example XIX. In this manner the formyloxy, acetoxy, butyryloxy and benzyloxy derivatives are prepared.

EXAMPLE XLIV Pyrido[2,3-d]Pyrimidin-4(3H)-One-2-Carboxamides

The procedure of Example IX is repeated but using the appropriate 2-aminonicotinamide as reactant in place of 2-aminonaphthalene-3-carboxamide to produce the amides corresponding to the esters of Examples XXXIX and XLIII.

EXAMPLE XLVI Pyrido[2,3-d]Pyrimidin-4(3H)-One-2-Hydroxamic Acids

The esters of Examples XXXIX and XLIII are converted by the procedure of Example XIV to hydroxamic acids of the formula: ##SPC7##

EXAMPLE XLVII

The ester products of Examples I, III-VI, VIII, X, XII, XIII, XVIII, XXVIII, XXXXIV-XXXVI, XXXIX, and XLIII are converted to the corresponding acids by the procedure of Example II-D, and to the disodium, dipotassium and diammonium salts by the procedure of Examples XXXIV-D and XXXIV-E.

The acids are converted to the calcium, magnesium, aluminum, triethylamine, tri-n-butylamine, piperidine, triethanolamine, diethylaminoethylamine, N,N'-dibenzylethylenediamine and pyrrolidine by reaction with an equivalent amount of the appropriate base (Ca(OH)₂, Mg(OH)₂, Al(OH)₃) or amine in water or ethanol followed by filtration of the salt if insoluble or by evaporation of the solvent if the salt is soluble therein.

EXAMPLE XLVIII Injectable Preparation

One thousand grams of ethyl pyrimido[4,5-b]quinolin-4(3H)-one-2-carboxylate are intimately mixed and ground with 2500 grams of sodium ascorbate. The ground, dry mixture is placed in vials and sterilized with ethylene oxide after which the vials are sterilely stoppered. For intravenous administration, sufficient water is added to the materials in the vials to form a solution containing 5.0 mg. of active ingredient per milliliter of injectable solution.

EXAMPLE XLIX Tablets

A tablet base is prepared by blending the following ingredients in the proportion by weight indicated:

    Sucrose, U.S.P.   80.3                                                         Tapioca Starch    13.2                                                         Magnesium Stearate                                                                               6.5                                                     

Into this tablet base there is blended sufficient ethyl 7,8-dimethoxypyrimido[4,5-b]quinolin-4(3H)-one-2-carboxylate to provide tablets containing 20, 100 and 250 mg. of active ingredient per tablet. The compositions are each compressed into tablets, each weighing 360 mg., by conventional means.

EXAMPLE L Capsules

A blend is prepared containing the following ingredients:

    Calcium carbonate, U.S.P.                                                                              17.6                                                   Dicalcium phosphate     18.8                                                   Magnesium trisilicate, U.S.P.                                                                          5.2                                                    Lactose, U.S.P.         5.2                                                    Potato starch           5.2                                                    Magnesium stearate A    0.8                                                    Magnesium stearate B    0.35                                              

To this blend is added sufficient ethyl 7,8-dimethoxypyrimido[4,5-b]quinolin-4(3H)-one-2-carboxylate to provide capsules containing 10, 25 and 50 mg. of active ingredient per capsule. The compositions are filled into conventional hard gelatin capsules in the amount of 350 mg. per capsule.

In like manner, capsules containing 2.0 mg. and 6.0 mg. of drug, ethyl 7,8-dimethoxypyrimido[4,5-b]quinoline-4-(3H)-one-2-carboxylate, and having 300 mg. of the following blends per capsule are prepared

    Ingredients        Weight mg/capsule                                           Drug               2.00                                                        N-methylglucamine  18.00                                                       Lactose, Anhydrous 241.20                                                      Corn Starch, Anhydrous                                                                            30.00                                                       *Talc              8.80                                                                           300.00                                                       *Talc added before encapsulation                                         

    Ingredients        Weight mg/capsule                                           Drug               6.00                                                        N-methylglucamine  18.00                                                       Lactose, Anhydrous 237.20                                                      Corn Starch, Anhydrous                                                                            30.00                                                       *Talc              8.80                                                                           300.00                                                 

EXAMPLE LI Suspension

A suspension of pyrimido[4,5-b]quinolin-4(3H)-one-2-carboxylic acid is prepared with the following composition:

    Effective ingredient                                                                               25.00 g.                                                   70% aqueous sorbitol                                                                               741.29 g.                                                  Glycerine, U.S.P.   185.35 g.                                                  Gum acacia (10% solution)                                                                          100.00 ml.                                                 Polyvinylpyrrolidone                                                                               0.50 g.                                                    Distilled water     sufficient to make                                                             1 liter                                               

To this suspension, various sweeteners and flavorants are added to improve the palatability of the suspension. The suspension contains approximately 25 mg. of effective agent per milliliter.

EXAMPLE LII

In like manner, the remaining products of this invention are formulated into tablets, capsules, solutions and suspensions.

EXAMPLE LIII Solution

A solution of ethyl 2-methylpyrimido[4,5-b]quinolin-4(3H)-one-2-carboxylate is prepared with the following composition:

    Effective ingredient                                                                              6.04 grams                                                  Magnesium chloride hexahydrate                                                                    12.36 grams                                                 Monoethanolamine   8.85 ml.                                                    Propylene glycol   376.00 grams                                                Water, distilled   94.00 ml.                                              

The resultant solution has a concentration of effective ingredient of 10 mg./ml. and is suitable for parenteral and, especially, for intramuscular administration.

EXAMPLE LIV Aerosol Suspension

A mixture of ethyl 7,8-dimethoxypyrimidin[4,5-b]quinolin-4(3H)-one-2-carboxylate (antiallergy agent) and the other ingredients under (a) in the examples below are micronized to a particle size of 1 to 5 microns in a ball mill. The resulting slurry is then placed in a container equipped with a valve and propellant (b) introduced by pressure filling through the valve nozzle to a gauge pressure of approximately 35-40 pounds per square inch at 20° C.

    ______________________________________                                         Suspension A            Percent                                                (a)  Antiallergy agent      0.25                                                    Isopropyl myristate    0.10                                                    Ethanol                26.40                                              (b)  60-40% mixture of      73.25                                                   1,2-dichlorotetrafluoroethane-                                                 1-chloropentafluoroethane                                                 Suspension B                                                                   (a)  Antiallergy agent      0.25                                                    Ethanol                26.50                                              (b)  A 60-40% mixture of    73.25                                                   1,2-dichlorotetrafluoroethane-                                                 1-chloropentafluoroethane.                                                ______________________________________                                    

EXAMPLE LV Aerosol Administration

An aqueous solution of ethyl 7,8-dimethoxypyrimido[4,5-b]quinolin-4-(3H)-one-2-carboxylic acid (Drug A, containing 3mg. of drug per ml. of solution) is placed in a standard nebulizer such as is available from the Vaponephrine Co., Edison, N. J. The solution is sprayed under an air pressure of 6 lbs. per square inch into a closed 8 × 8 × 12 inch plastic container for 6 minutes. The container has four openings to accommodate the heads of four rats. Four rats are exposed to the drug simultaneously with only their heads coming in contact with the aerosol. Disodium cromoglycate (Drug B, 50 mg./ml.) is used as a basis for comparison. The results obtained in the PCA reaction above are tabulated below:

                     Time Interval between                                                                           % Animals                                    Drug  Concn.     Drug and Challenge                                                                              Protected                                    ______________________________________                                         A     3 mg/ml.   0 min.           82                                           A     3 mg./ml.  5 min.           56                                           B     50 mg./ml. 0 min.           0                                            B     50 mg./ml. 5 min.           0                                            ______________________________________                                    

PREPARATION A 2-Amino-5-Alkylnicotinamides

The following 2-amino-5-alkylnicotinamides are produced from the corresponding 2-amino-5-alkylnicotinonitriles via treatment with alkaline hydrogen peroxide according to the procedure of Taylor et al., J. Org. Chem. 19, 1633-9 (1954). ##SPC8##

R₃

c₂ h₅

n-C₃ H₇

i-C₃ H₇

n-C₄ H₉

t-C₄ H₉

s-C₄ H₉

PREPARATION B 2-Amino-5-Halonicotinic Acids and Amides A. 2-Amino-5-chloronicotinic acid and hydrochloride

A suspension of 40.0 g. of 2-aminonicotinic acid in 2 l. of acetic acid is stirred while chlorine gas is bubbled through the reaction mixture at a moderate rate for 1.25 hours. The resulting solution is allowed to stir at ambient temperatures for 20 hours, and is then treated with an equal volume of diethyl ether. The resulting precipitate of 2-amino-5-chloronicotinic acid hydrochloride is filtered, washed with ether and dried in vacuo at 80° C. for several hours, 51.0 g., m.p. 251° C. (dec.).

The free acid is obtained by treating a cold aqueous solution of the hydrochloride salt with sufficient ammonium hydroxide to provide a pH 5. The resulting product is filtered, washed with a small amount of acetone and dried in vacuo.

B. 2-Amino-5-bromonicotinic acid and hydrobromide

2-Aminonicotinic acid (3.6 g.) in 450 ml. of acetic acid is treated dropwise over a period of 10-15 minutes with 4 ml. of bromine in 50 ml. of acetic acid. The reaction mixture is allowed to stir at room temperature for 1.5-2 hours, and is then diluted with 2 liters of diethyl ether. The precipitate which forms, 2-amino-5-bromonicotinic acid hydrobromide, is filtered and dried, 5.6 g., m.p. 280° C. (dec.).

The free acid is liberated by adding sufficient ammonium hydroxide to an aqueous solution of the hydrobromide salt to provide a solution of pH 5. The resulting free acid is filtered from the cooled mixture and dried in vacuo.

C. 2-Amino-5-chloro-6-methylnicotinic acid hydrochloride

In a manner similar to that of Preparation B-A, 2-amino-6-methylnicotinic acid is chlorinated in acetic acid to provide the desired product.

D. 2-Amino-4-methyl-5-chloronicotinic acid hydrochloride

3-Cyano-4-methylpyridine, Webb et al., J. Am. Chem. Soc., 66, 1456 (1944) is converted via the sequences of Taylor et al., J. Org. Chem., 19, 1633 (1954) to 2-amino-4-methylnicotinic acid, which under the conditions of Preparation B-A is chlorinated at the 5-position to provide the desired intermediate, 2-amino-4-methyl-5-chloronicotinic acid hydrochloride.

E. 2-Amino-4-methyl-5-bromonicotinic acid hydrobromide

In a manner analogous to that of Preparation B-B, 2-amino-4-methylnicotinic acid is brominated in an acetic acid solvent to provide the desired compound in good yield.

F. 2-Amino-4,6-dimethyl-5-chloronicotinic acid hydrochloride

Chlorine gas is bubbled through a solution of 1.89 g. of 2-amino-4,6-dimethylnicotinic acid hydrochloride in 150 ml. of acetic acid at 36° c. for 20-25 minutes. The resulting solid is filtered, washed with diethyl ether and dried, 1.29 g., m.p. 232°-234° C. (dec.).

G. 2-Amino-5-chloro-6- and 4-methylnicotinamide

Fifty grams of 2-amino-5-chloro-6-methylnicotinic acid hydrochloride is added in small portions to 185 ml. of cold (3° C.) acetyl chloride containing 80 g. of phosphorous pentachloride and the resulting reaction mixture allowed to stir at room temperature for 16 hours. The precipitate which forms is filtered, washed with 150 ml. of methylene chloride and partially dissolved in 1.2 liters of acetonitrile. While the solution of 2-amino-5-chloronicotinic acid chloride is being stirred at ambient temperatures, ammonia gas is bubbled through the slurry for 40 min. at a moderate rate. The solids are filtered and the residue, remaining after the filtrate is concentrated to dryness, is partially dissolved in acetone. The acetone suspension is filtered and the filtrate concentrated in vacuo to give 13.9 g. of the desired nicotinamide, m.p. 227°-229° C.

In like manner the remaining nicotinic acid derivatives described above are converted to the corresponding amides.

PREPARATION C 2-Amino-5-Alkylnicotinonitriles 2-Amino-4-methyl-5-ethylnicotinonitrile

To 67.0 g. (0.5 mole) of 1-methylbutylidene malononitrile (Cope, et al., J. Am. Chem. Soc., 63, 733 (1941) is added 260 ml. of acetic anhydride and 50 ml. of triethyl orthoformate and the resulting solution heated to reflux for 18 hours. The reaction mixture is concentrated under reduced pressure, and the residue is dissolved in 300 ml. of acetonitrile. Ammonia gas is bubbled through the reaction mixture at a moderate rate for 2 hours and the mixture then heated to reflux for 4 hours. The solvent is removed from the filtered mixture under reduced pressure and the residual material triturated with diethyl ether. Filtration and drying give the desired crude product.

The above reaction procedure is repeated, employing the indicated malononitrile and ortho ester starting materials to provide the corresponding substituted 2-aminonicotinonitriles: ##STR19##Starting Materials ProductMalononitrile ortho ester R₄ R₃ R₂__________________________________________________________________________CH₃ CH₂ CH₂ C(CH₃)=C(CN)₂ CH₃ C(OC₂ H₅)₃ CH₃ -- C₂ H₅ -- CH₃ --CH₃ (CH₂)₃ CH=C(CN)₂ CH₃ C(OC₂ H₅)₃ CH₃ n-C₃ H₇ -- H--CH₃ (CH₂)₃ C(CH₃)=C(CN)₂ CH₃ C(OC₂ H₅)₃ CH₃ -- n-C₃ H₇ -- CH₃ --(CH₃)CHCH₂ C(CH₃)=C(CN)₂ CH₃ C(OC₂ H₅)₃ CH₃ i-C₃ H₇ -- CH₃ --CH₃ (CH₂)₄ CH=C(CN)₂ CH₃ C(OC₂ H.sub. 5)₃ CH₃ -- n-C₄ H₉ -- H--CH₃ CH(C₂ H₅)CH₂ C(CH₃)=C(CN)₂ HC(OC₂ H₅)₃ H-- s-C₄ H₉ -- CH₃ --(CH₃)₃ CCH₂ C(CH₃)=C(CN)₂ CH₃ C(OC₂ H₅)₃ CH₃ t-C₄ H₉ -- CH₃ --__________________________________________________________________________

The nitriles are converted to the corresponding amides by the procedure of Taylor et al., J. Org. Chem. 19, 1633-9 (1954).

PREPARATION D 2-Amino-4-Substituted-Quinoline-3-Carboxamides

The appropriate substituted 2-aminobenzophenone or alkyl-(2-aminophenyl)ketone of the formula ##SPC9##

wherein the R variables have the values given below, and malononitrile are mixed together in pyridine in a 1:2 molar ratio and heated under reflux for 24 hours. Sufficient pyridine is used to achieve solution of the reactants. The mixture is then cooled and concentrated in vacuo. The precipitate is collected, washed with ethanol and recrystallized from ethanol.

The thus-obtained nitrile is hydrolyzed by heating in excess of 95% sulfuric acid for three hours on a steam bath. The mixture is then poured into a large volume of ice water and allowed to stand overnight. The aqueous mixture is filtered if necessary and the filtrate made alkaline (pH 11-11.5) with 5N sodium hydroxide. The amide product precipitates and is recovered by filtration. It is washed with water and dried.

The following compounds are thus prepared: ##STR20##R₂ R₃ R₄ R₅ R₁______________________________________H H H Cl C₆ H₅H Cl H H C₆ H₅H Br H H C₆ H₅H F H H C₆ H₅H H CH₃ H C₆ H₅H Cl Cl H C₆ H₅H CH₃ CH₃ H C₆ H₅H OCH₃ H H C₆ H₅H H OCH₃ H C₆ H₅H CH₃ Cl H C₆ H₅H H CH₃ CH₃ C₆ H₅H Br H H C₂ H₅H H Cl H C₂ H₅H OCH₃ H H C₂ H₅H OCH₃ OCH₃ H C₂ H₅H H H H CH₃H H H H C₂ H₅H H H H n-C₃ H₇H H H H n-C₄ H₉H H Br H CH₃H Cl H H CH₃H H H OCH₃ CH₃H OCH₃ OCH₃ H CH₃H Cl CH₃ H CH₃H H OCH₃ OCH₃ CH₃H H OC₂ H₅ OC₂ H₅ CH₃H CH₃ CH₃ H CH₃H H I H CH₃H Cl H H n-C₃ H₇H Cl H H n-C₄ H₉CH₃ CH₃ CH₃ OCH₃ CH₃H OCH₃ H H CH₃______________________________________

PREPARATION E α-Cyano-β-(2-Nitro-MethylthiophenylAcrylamides

The following compounds are prepared by reaction of the appropriate α-Cyano-β-(2-nitro-chlorophenyl)acrylamide with sodium methylmercaptide or sodium benzylmercaptide according to the procedure of Example XXI-A. ##STR21##R₂ R₃ R₄ R₅______________________________________CH₃ S H H HH CH₃ S CH₃ S HCH₃ S H H CH₃ SH H CH₃ S HC₆ H₅ S H H HC₆ H₅ S H H C₆ H₅ SH C₆ H₅ S C₆ H₅ S HH C₆ H₅ S H H______________________________________

PREPARATION F

Reaction of the appropriate chloro substituted 2-aminonaphthalene-3-carboxamide or the appropriate chloro substituted 2-aminonicotinamide with sodium methylmercaptide, sodium benzylmercaptide, sodium benzylate or the appropriate sodium alcoholate according to the procedure of Example XXI-A affords the following compounds: ##STR22##R₂ R₃ R₄ R₂ R₃ R₄______________________________________SCH₃ H H H OC₂ H₅ HH SCH₃ H H O--n-C₄ H₉ HH H SCH₃ H OC₇ H₇ HSC₇ H₇ H H OC₇ H₇ H HH SC₇ H₇ HH H SC₇ H₇______________________________________ ##STR23##R₂ R₃ R₄ R₂ R₃ R₄______________________________________H SCH₃ H CH₃ OC₂ H₅ HCH₃ SCH₃ H CH₃ O--n-C₄ H₉ CH₃CH₃ SCH₃ CH₃ CH₃ OC₇ H₇ HCH₃ SC₇ H₇ H OC₇ H₇ H HCH₃ SC₇ H₇ CH₃______________________________________

PREPARATION G Benzyl Ethers of Hydroxy Substituted Benzaldehydes

A mixture of the appropriate hydroxy substituted benzaldehyde (0.66 mole), absolute ethanol (450 ml.), anhydrous potassium carbonate (91.5g.) and freshly distilled benzyl chloride (95.6g., 0.76 mole) is refluxed for 2-3 days. The reaction mixture is filtered to remove the salts and the filtrate concentrated under reduced pressure. The oily residue is cooled and the resulting solid is recrystallized from an appropriate solvent such as ethanol.

In this manner, the following benzyl ethers are prepared: ##STR24##R₂ R₃ R₄ R₅______________________________________H H OC₇ H₇ HOC₇ H₇ OCH₃ H HH OC₇ H₇ OC₇ H₇ HOC₇ H₇ H H HOC₇ H₇ Br H BrH OCH₃ OC₇ H₇ BrH OCH₃ OC₇ H₇ OCH₃OC₇ H₇ OCH₃ OCH₃ HH Br H OC₇ H₇H H H OC₇ H₇C₂ H₅ OC₇ H₇ H HC₂ H₅ H OC₇ H₇ HH OC₇ H₇ OC₂ H₅ HH OC₂ H₅ OC₇ H₇ HH F OC₇ H₇ HH Br OC₇ H₇ HH Cl OC₇ H₇ HH OC₇ H₇ H OCH₃CH₃ CH₃ OC₇ H₇ CH₃CH₃ OC₇ H₇ CH₃ CH₃H i-C₃ H₇ OC₇ H₇ i-C₃ H₇______________________________________

PREPARATION H 5-Benzyloxy-4-Methoxy-2-Nitrobenzaldehyde

To concentrated nitric acid (200 ml.) maintained at 0°C. is added 3-benzyloxy-4-methoxybenzaldehyde (48 g., 0.198 mole) over a 30 minute period with stirring. When addition is complete, the temperature is allowed to rise to 15°C. and stirring continued for another 30 minutes. The mixture is then added to ice-water and the yellow precipitate collected by filtration and dried. Yield = 52.8g. (94%); m.p. 131°-132°C.

In like manner, the products of Preparation G are nitrated to their 2-nitro derivatives. In those cases wherein a mixture of products is obtained the products are separated by chromatography on an acid-washed silica using an appropriate solvent such as benzene or chloroform. 

What is claimed is:
 1. A compound selected from the group consisting of ##SPC10##and the pharmaceutically acceptable cation salts thereof wherein R is selected from the group consisting of methyl, ethyl, acetyl and COR^(o) wherein R^(o) is selected from the group consisting of hydroxy, alkoxy having 1 to 4 carbon atoms, hydroxyalkoxy having 2 to 4 carbon atoms, amino and hydroxyamino; Y is selected from the group consisting of (a) hydrogen, and (b) alkyl having 1 to 4 carbon atoms, carbalkoxyalkyl having 2 to 5 carbon atoms in the carbalkoxy moiety and 1 to 4 carbon atoms in the alkyl moiety, carboxyalkyl having 1 to 4 carbon atoms in the alkyl moiety, --(CH₂)_(m) -O-CO-C₆ H₅ and --(CH₂)_(m) -O-CO-alkyl having 1 to 4 carbon atoms in the alkyl moiety; with the provisos that when R is methyl or ethyl, Y is selected from group (b); and when R is COR^(o) wherein R^(o) is amino or hydroxyamino, Y is hydrogen; m is an integer from 2 up to and including 4; R₁ is selected from the group consisting of hydrogen, alkyl having 1 to 4 carbon atoms and phenyl; each of R₂, R₃, R₄ and R₅ is selected from the group consisting of hydrogen, alkyl having 1 to 4 carbon atoms, alkoxy having 1 to 4 carbon atoms, halo, benzyloxy, hydroxyl, thiol, alkanoyloxy having 1 to 4 carbon atoms, benzylthio, benzoyloxy, benzylsulfinyl, methylthio and methylsulfinyl; and R₂ and R₃, and R₃ and R₄ when taken together are selected from the group consisting of methylenedioxy and ethylenedioxy.
 2. A compound according to claim 1 wherein each of R₁, R₂, R₃, R₄, Y and R₅ is hydrogen; and R is COR^(o) wherein R^(o) is said alkoxy.
 3. A compound according to claim 1 wherein each of R₁, R₂, R₅, and Y is hydrogen; R is COR^(o) ; and each of R^(o), R₃ and R₄ is said alkoxy.
 4. A compound according to claim 1 wherein each of R₁, R₂, R₅ and Y is hydrogen; R is COR^(o) wherein R^(o) is hydroxy; and each of R₃ and R₄ is said alkoxy.
 5. A compound according to claim 1 wherein each of R₁, R₂, R₅, and Y is hydrogen; R is COR^(o) wherein R^(o) is said alkoxy; one of R₃ and R₄ is hydroxy and the other is said alkoxy.
 6. A compound according to claim 3 wherein R^(o) is ethoxy; and each of R₃ and R₄ is methoxy.
 7. A compound according to claim 4 wherein each of R₃ and R₄ is methoxy.
 8. A compound according to claim 5 wherein R^(o) is n-butoxy; R₃ is hydroxy; and R₄ is methoxy.
 9. A compound according to claim 5 wherein R^(o) is ethoxy; R₃ is methoxy; and R₄ is hydroxy. 